Quantitative Packaging of Active Enzymes into a Protein Cage

被引：105
作者：
Azuma, Yusuke [1 ]
Zschoche, Reinhard [1 ]
Tinzl, Matthias [1 ]
Hilvert, Donald [1 ]
机构：
[1] ETH, Organ Chem Lab, CH-8093 Zurich, Switzerland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 04期
基金：
欧洲研究理事会;
关键词：
enzymes; host-guest systems; nanoreactors; protein engineering; synthetic biology; VIRUS-LIKE PARTICLES; BACTERIOPHAGE P22; ENCAPSULATION; PURIFICATION; NANOREACTORS; EXPRESSION; EVOLUTION; SUBSTRATE; CATALYSIS; CONTAINER;
D O I：
10.1002/anie.201508414
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Genetic fusion of cargo proteins to a positively supercharged variant of green fluorescent protein enables their quantitative encapsulation by engineered lumazine synthase capsids possessing a negatively charged lumenal surface. This simple tagging system provides a robust and versatile means of creating hierarchically ordered protein assemblies for use as nanoreactors. The generality of the encapsulation strategy and its effect on enzyme function were investigated with eight structurally and mechanistically distinct catalysts.
引用
收藏
页码：1531 / 1534
页数：4
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