DEVELOPMENT OF A VALIDATED METHOD FOR SIMULTANEOUS QUANTIFICATION OF CLARITHROMYCIN AND LEVOFLOXACIN BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTOR

This study aims at the development of a validated reverse phase-high performance liquid chromatography (RP-HPLC) method for the simultaneous quantification of levofloxacin (LVX) and clarithromycin (CAM). Eclipse X DB C18 column (5 mu m, 4.6 x250 mm) was used as a stationary phase, and monobasic potassium phosphate (KH2PO4) buffer (0.035 M): acetonitrile (75 : 25) was used as an isocratic mobile phase. The pH of the mobile phase was adjusted to 4.5 with the help of diluted orthophosphoric acid. The diode array detector (DAD) was operated at 205 nm and 294 nm for the detection of CAM and LVX, respectively. Limit of detection (LOD) values for CAM and LVX were 1.22 mu g/mL and 0.79 mu g/mL, respectively. Limit of quantification(LOQ) values for CAM and LVX were 4.08 mu g/mL and 2.64 mu g/mL, respectively. The method was precise as% relative standard deviation (% RSD) values were less than 2% for all types of precision. This method was successfully applied to determine the percentage contents of both drugs in the pharmaceutical preparation.