Purification and characterization of dermatan sulfate from the skin of the eel, Anguilla japonica

被引：34

作者：

Sakai, S

Kim, WS

Lee, IS

Kim, YS

Nakamura, A

Toida, T ^{[1
]}

Imanari, T

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Bioanalyt Chem, Chiba 2638522, Japan

[2] Seoul Natl Univ, Coll Pharm, Nat Prod Res Inst, Seoul 110460, South Korea

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 03期

关键词：

delmatan sulfate; eel skin; Anguilla japonica; H-1; NMR; anti-factor; IIa activity;

D O I：

10.1016/S0008-6215(02)00442-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosaminoglycans were isolated from the eel skin (Anguilla japonica) by actinase and endonuclease digestions, followed by a beta-elimination reaction and DEAE-Sephacel chromatography. Dermatan sulfate was the major glycosaminoglycan in the eel skin with 88% of the total uronic acid. The content of the IdoA2Salpha1-->4GaINAc4S sequence in eel skin, which shows anticoagulant activity through binding to heparin cofactor II, was two times higher than that of dermatan sulfate from porcine skin. The anti-IIa activity of eel skin dermatan sulfate was determined to be 2.4 units/mg, whereas dermatan sulfate from porcine skin shows 23.2 units/mg. The average molecular weight of dermatan sulfate was determined by gel chromatography on a TSKgel G3000SWXL column as 14 kDa. Based on H-1 NMR spectroscopy, the presence of 3-sulfated and/or 2,3-sulfated IdoA residues was suggested. The reason why highly sulfated dermatan sulfate does not show anticoagulant activity is discussed. In addition to dermatan sulfate, the eel skin contained a small amount of keratan sulfate, which was identified by keratanase treatment. (C), 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：263 / 269

页数：7

共 51 条

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