Transgenerational inheritance of susceptibility to diabetes-induced male subfertility

被引：22
作者：
Pavlinkova, Gabriela [1 ]
Margaryan, Hasmik [2 ]
Zatecka, Eva [2 ]
Valaskova, Eliska [2 ]
Elzeinova, Fatima [2 ]
Kubatova, Alena [2 ]
Bohuslavova, Romana [1 ]
Peknicova, Jana [2 ]
机构：
[1] BIOCEV, Inst Biotechnol CAS, Lab Mol Pathogenet, Vestec, Czech Republic
[2] BIOCEV, Inst Biotechnol CAS, Lab Reprod Biol, Vestec, Czech Republic
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
HIGH-FAT DIET; GENE-EXPRESSION; IN-UTERO; SPERM; INSULIN; STREPTOZOTOCIN; MELLITUS; OBESITY; HYPOMETHYLATION; ABNORMALITIES;
D O I：
10.1038/s41598-017-05286-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality. The testicular tissue of diabetic males showed significant tissue damage, disruption of meiotic progression, and changes in the expression of genes encoding proteins important for spermiogenesis. Paternal diabetes altered sperm quality and expression pattern in the testes in offspring of two subsequent generations. Our study revealed that paternal diabetes increased susceptibility to infertility in offspring through gametic alternations. Our data also provide a mechanistic basis for transgenerational inheritance of diabetes-associated pathologies since protamines may be involved in epigenetic regulations.
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页数：14
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