Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media

被引:25
作者
Citiulo, Francesco [1 ]
Moran, Gary P. [1 ]
Coleman, David C. [1 ]
Sullivan, Derek J. [1 ]
机构
[1] Univ Dublin, Trinity Coll Dublin, Div Oral Biosci, Microbiol Res Unit,Dublin Dent Sch & Hosp, Dublin 2, Ireland
关键词
chlamydospore; Candida albicans; Candida dubliniensis; ELECTRON-MICROSCOPY; STAIB AGAR; GROWTH; DIFFERENTIATION; MORPHOGENESIS;
D O I
10.1111/j.1567-1364.2009.00533.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Candida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are > 30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.
引用
收藏
页码:1051 / 1060
页数:10
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