A new method to evaluate macaque health using exhaled breath: A case study of M-tuberculosis in a BSL-3 setting

被引：24

作者：

Mellors, Theodore R. ^{[1
]}

Blanchet, Lionel ^{[1
]}

Flynn, JoAnne L. ^{[2
]}

Tomko, Jaime ^{[2
]}

O'Malley, Melanie ^{[2
]}

Scanga, Charles A. ^{[2
]}

Lin, Philana L. ^{[3
]}

Hill, Jane E. ^{[1
]}

机构：

[1] Dartmouth Coll, Sch Engn, 14 Engn Dr, Hanover, NH 03755 USA

[2] Univ Pittsburgh, Sch Med, Dept Microbiol & Mol Genet, Pittsburgh, PA USA

[3] UPMC, Childrens Hosp, Div Infect Dis, Dept Pediat, Pittsburgh, PA USA

来源：

JOURNAL OF APPLIED PHYSIOLOGY | 2017年 / 122卷 / 03期

基金：

美国国家卫生研究院;

关键词：

breath; tuberculosis; GCxGC-TOFMS; macaque; VOLATILE ORGANIC-COMPOUNDS; SOLID-PHASE MICROEXTRACTION; MASS-SPECTROMETRY; LUNG-CANCER; PULMONARY TUBERCULOSIS; X GC; BIOMARKERS; AIR; NORMALIZATION; INFECTION;

D O I：

10.1152/japplphysiol.00888.2016

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Breath is hypothesized to contain clinically relevant information, useful for the diagnosis and monitoring of disease, as well as understanding underlying pathogenesis. Nonhuman primates, such as the cynomolgus macaque, serve as an important model for the study of human disease, including over 70 different human infections. In this feasibility study, exhaled breath was successfully collected in less than 5 min under Biosafety Level 3 conditions from five anesthetized, intubated cynomolgus and rhesus macaques, before and after lung infection with M. tuberculosis. The breath was subsequently analyzed using comprehensive two-dimensional gas chromatography coupled to time-offlight mass spectrometry. A total of 384 macaque breath features were detected, with hydrocarbons being the most abundant. We provide putative identification for 19 breath molecules and report on overlap between the identified macaque breath compounds and those identified in previous human studies.

引用

页码：695 / 701

页数：7