Identification of genes differentially expressed in C6 glioma cells transfected with connexin43

被引：46
作者：
Naus, CCG [1 ]
Bond, SL
Bechberger, JF
Rushlow, W
机构：
[1] Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5C1, Canada
[2] Univ Western Ontario, Dept Psychiat, London, ON N6A 5C1, Canada
来源：
BRAIN RESEARCH REVIEWS | 2000年 / 32卷 / 01期
基金：
英国医学研究理事会;
关键词：
gap junction; gene expression; differential display; growth control;
D O I：
10.1016/S0165-0173(99)00087-9
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Astrocytes are characterized by extensive gap junctional intercellular communication (GJIC) mediated primarily by channels composed of connexin43. Tn contrast, C6 glioma cells are deficient in connexin expression and gap junctional communication. Transfection of these glioma cells with connexin cDNAs results in changes in cellular phenotype following increased GJIC. Specifically, connexin expression correlates with reduced cellular proliferation and tumorigenicity, To characterize the role of gap junctions in this growth control, we have screened for changes in gene expression by differential display. We have observed that these changes in GJIC are associated with changes in expression of several genes, including those coding for a number of secreted factors which may play a role in modulating the tumor phenotype of these cells. These include the immediate early gene cyr61, ostoepontin and the KC gene (murine homologue of the human gro gene). (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：259 / 266
页数：8
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