Live-cell STED nanoscopy of mitochondrial cristae

被引：140

作者：

Stephan, Till ^{[1
,3
]}

Roesch, Axel ^{[1
,3
]}

Riedel, Dietmar ^{[2
]}

Jakobs, Stefan ^{[1
,3
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Lab Electron Microscopy, D-37077 Gottingen, Germany

[3] Univ Med Ctr Gottingen, Clin Neurol, D-37075 Gottingen, Germany

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

SUPERRESOLUTION MICROSCOPY; FLUORESCENCE; MEMBRANE; FUSION;

D O I：

10.1038/s41598-019-48838-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They exhibit a smooth outer membrane and a highly convoluted inner membrane that forms invaginations called cristae. Imaging cristae in living cells poses a formidable challenge for super-resolution light microscopy. Relying on a cell line stably expressing the mitochondrial protein COX8A fused to the SNAP-tag and using STED (stimulated emission depletion) nanoscopy, we demonstrate the visualization of cristae dynamics in cultivated human cells. We show that in human HeLa cells lamellar cristae are often arranged in groups separated by voids that are generally occupied by mitochondrial nucleoids.

引用

页数：6

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