Gold nanoparticles as a label-free probe for the detection of amyloidogenic protein

被引：20
|
作者：
Zhang, Hai-Jie [1 ]
Zheng, Hu-Zhi [1 ]
Long, Yi-Juan [1 ]
Xiao, Geng-Fu [2 ]
Zhang, Ling-Yan [1 ]
Wang, Qin-Long [1 ]
Gao, Mei [1 ]
Bai, Wen-Jun [1 ]
机构：
[1] Southwest Univ, Coll Chem & Chem Engn, Chongqing 400715, Peoples R China
[2] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
来源：
TALANTA | 2012年 / 89卷
基金：
中国国家自然科学基金;
关键词：
Amyloidogenic protein; Gold nanoparticle; Prion protein; Label-free detection; CREUTZFELDT-JAKOB-DISEASE; PRION PROTEIN; CAPILLARY-ELECTROPHORESIS; COLORIMETRIC DETECTION; CEREBROSPINAL-FLUID; PARKINSONS-DISEASE; ALZHEIMERS-DISEASE; ALPHA-SYNUCLEIN; QUANTUM DOTS; BLOOD;
D O I：
10.1016/j.talanta.2011.12.052
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Because amyloidogenic proteins, such as prion protein, beta-amyloid peptide and alpha-synuclein, are associated with a variety of diseases, methods for their detection are important. Recombinant prion protein (rPrP) can selectively induce aggregation of dihydrolipoic acid capped gold nanoparticles (DHLA-AuNPs), which reduces the absorbance of the DHLA-AuNPs and changes their color from red to blue. These changes were used for label-free qualitative and quantitative detection of amyloidogenic protein. The addition of NaCl improved the detection sensitivity considerably, and the detection limit was as low as 33 pmol/L. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：401 / 406
页数：6
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