In vitro profiling of endocrine disrupting potency of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47) and related hydroxylated analogs (HO-PBDEs)

被引：36
作者：
Liu, Hongling [1 ]
Hu, Wei [1 ]
Sun, Hong [2 ]
Shen, Ouxi [2 ]
Wang, Xinru [2 ]
Lam, Michael H. W. [3 ]
Giesy, John P. [1 ,3 ,4 ,5 ,6 ,7 ]
Zhang, Xiaowei [1 ]
Yu, Hongxia [1 ]
机构：
[1] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China
[2] Nanjing Med Univ, Inst Toxicol, Key Lab Reprod Med, Nanjing 210029, Peoples R China
[3] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
[4] Univ Saskatchewan, Dept Biomed & Vet Biosci, Saskatoon, SK, Canada
[5] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada
[6] Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA
[7] Michigan State Univ, Dept Zool, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
来源：
MARINE POLLUTION BULLETIN | 2011年 / 63卷 / 5-12期
关键词：
Receptor reporter gene assay; BDE47; Metabolite; Endocrine disruption; POLYBROMINATED DIPHENYL ETHERS; BROMINATED FLAME RETARDANTS; AROMATASE CYP19 ACTIVITY; ESTROGEN-RECEPTOR-ALPHA; PEARL RIVER ESTUARY; THYROID-HORMONE; POLYCHLORINATED-BIPHENYLS; ORGANOCHLORINE PESTICIDES; EXPOSURE; METABOLITES;
D O I：
10.1016/j.marpolbul.2011.04.019
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The potential of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) and its related hydroxylated analogs (2'-HO-BDE28, 6-HO-BDE47, 4'-HO-BDE17, and 4'-HO-BDE49) to modulate estrogen/thyroid/androgen receptor-(ER, TR, AR), mediated responses were investigated by use of reporter gene assays. Exposure to 1 or 10 mu M, 4'-HO-BDE17 significantly up-regulated expression of Luc, whereas other four chemicals did not induce Luc expression under control of the ER. Anti-estrogenic potency was observed for 4'-HO-BDE17 (IC50 = 1.14 mu M) > 6-HO-BDE47 (IC50 = 2.65 mu M) > 2'-HO-BDE28 (IC50 = 9.49 mu M) > BDE47 (IC50 = 21.11 mu M). No anti-estrogenic effect of 4'-HO-BDE49 was observed. Both 4'-HO-BDE17, 4'-HO-BDE49 resulted in greater responses of Luc expression induced by T3. BDE47, 2'-HO-BDE28, 6-HO-BDE47 did not show any effect on the expression of Luc induced by 5 nM T3. 6-HO-BDE47 (IC50 = 0.34 mu M) > 4'-HO-BDE17 (IC50 = 1.41 mu M) > BDE47 (IC50 = 3.83 mu M) > 2'-HO-BDE28 (IC50 = 29.22 mu M) exhibited anti-androgenic potency, while 4'-HO-BDE49 did not show androgenic transcriptional activity. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：287 / 296
页数：10
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