Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

被引：49

作者：

Su, Pu ^{[1
]}

Liu, Nan ^{[1
]}

Zhu, Maoxiang ^{[2
]}

Ning, Baoan ^{[1
]}

Liu, Ming ^{[1
]}

Yang, Zhihua ^{[2
]}

Pan, Xiujie ^{[2
]}

Gao, Zhixian ^{[1
]}

机构：

[1] Tianjin Key Lab Risk Assessment & Control Technol, Inst Hlth & Environm Med, Tianjin 300050, Peoples R China

[2] Inst Radiat Med, Beijing 100850, Peoples R China

来源：

TALANTA | 2011年 / 85卷 / 02期

关键词：

Suspension array; xMAP; Antibiotics; Milk; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; RAW-MILK; COWS MILK; RESIDUES; GENTAMICIN; CHLORAMPHENICOL; IMMUNOASSAYS; TYLOSIN;

D O I：

10.1016/j.talanta.2011.05.040

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new suspension array technology is proposed for the simultaneous quantitative determination of five antibiotics tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol in milk. A novel treatment of milk samples for suspension array with diethyl ether was performed which greatly reduced the interference of the disturbing components in milk on the reaction results with no significant effect on detection sensitivity. Compared with using biotin labeled monoclonal antibody, using of secondary antibody-biotin make the detection sensitivity further improved. The minimum detectable concentration in samples of tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol were 0.3, 1.5, 4, 20, and 25 ng/ml, respectively, and the working ranges of samples were 6-400, 7-300, 8-200, 90-3000 and 70-8000 ng/ml, respectively. The mean recovery was 89.38-113.73% with a standard deviation within 16.62%. The suspension assay technology is powerful for the fast quantitative analysis of multi-antibiotics residue in milk. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1160 / 1165

页数：6

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