Detection limit enhancement of antiarrhythmic drugs in human plasma using capillary electrophoresis with dispersive liquid-liquid microextraction and field-amplified sample stacking method

被引：21

作者：

Fazeli-Bakhtiyari, Rana ^{[1
,2
]}

Sorouraddin, Mohammad Hossein ^{[2
]}

Farajzadeh, Mir Ali ^{[2
]}

Jouyban, Abolghasem ^{[3
,4
]}

机构：

[1] Tabriz Univ Med Sci, Liver & Gastrointestinal Dis Res Ctr, Tabriz, Iran

[2] Univ Tabriz, Dept Analyt Chem, Fac Chem, Tabriz, Iran

[3] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[4] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

来源：

BIOANALYSIS | 2015年 / 7卷 / 01期

关键词：

SOLID-PHASE EXTRACTION; CHROMATOGRAPHY-MASS-SPECTROMETRY; SUPERCRITICAL-FLUID EXTRACTION; BETA-BLOCKER DRUGS; HUMAN URINE; ELECTROKINETIC CHROMATOGRAPHY; CARDIOVASCULAR DRUGS; ZONE-ELECTROPHORESIS; SOLVENT STACKING; VERAPAMIL;

D O I：

10.4155/bio.14.175

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: A new capillary zone electrophoresis (CZE) with ultraviolet detection method has been developed and validated for the analysis of four antiarrhythmic drugs in human plasma samples. Methods: In this study, a dispersive liquid-liquid microextraction (DLLME) coupled with field-amplified sample stacking (FASS) was employed for biological samples clean-up and sensitivity enhancement in CZE. Results: Under optimum DLLME-FASS-CZE conditions, enhancement factors were in the range of 157-314. The method was validated over the concentration range of 20-800 ng/ml in human plasma. Inter- and intra-day precision and the accuracy were less than 20%; the detection limits ranged from 2.5 to 4.7 ng/ml. Furthermore, the validated method was successfully applied to the detection of studied drugs in patients' plasma samples.

引用

页码：21 / 37

页数：17

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