Measurement of the concentration of serum soluble interleukin-2 receptor alpha chain in dogs with lymphoma

被引：3

作者：

Mizutani, Noriyuki ^{[1
]}

Goto-Koshino, Yuko ^{[1
]}

Kurata, Keigo ^{[2
]}

Fujiwara-Igarashi, Aki ^{[3
]}

Sakaguchi, Masahiro ^{[4
]}

Asada, Minoru ^{[5
]}

Ohno, Koichi ^{[1
]}

Tsujimoto, Hajime ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

[2] Inst Tokyo Environm Allergy, Bunkyo Ku, 1-2-5 Yushima, Tokyo 1130034, Japan

[3] Nippon Vet & Life Sci Univ, Dept Clin Vet Med, Div Therapeut Sci 1, 1-7-1 Kyonan Cho, Musashino, Tokyo 1830023, Japan

[4] Azabu Univ, Sch Vet Med, Lab Vet Microbiol 1, Chuo Ku, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 2525201, Japan

[5] Nippon Med Sch, Grad Sch Med, Dept Pharmacol, Bunkyo Ku, 1-1-5 Sendagi, Tokyo 1138602, Japan

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2020年 / 225卷

基金：

日本学术振兴会;

关键词：

CD25; Dog; Interleukin-2 receptor alpha; Lymphoma; Soluble interleukin-2 receptor; NON-HODGKINS-LYMPHOMA; CANINE MALIGNANT-LYMPHOMAS; B-CELL LYMPHOMA; CLINICAL-FEATURES; RECEPTOR; EXPRESSION; LEVEL; PROTOCOL; CLASSIFICATION; CHEMOTHERAPY;

D O I：

10.1016/j.vetimm.2020.110054

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Soluble interleukin-2 receptor (sIL-2r) is released directly from the surface of lymphocytes expressing interleukin-2 receptor alpha chain (CD25), and its serum concentration has been found to reflect the prognosis of human lymphoproliferative malignancies. In this study, we demonstrated the presence of sIL-2r in canine serum and developed a specific sandwich enzyme-linked immunosorbent assay (ELISA) to quantify the concentration of canine serum sIL-2r. In the immunoprecipitation (IP) assay, CD25 protein weighing approximately 45 kDa was detected in canine serum, smaller than the membrane-bound CD25 (approximately 55 kDa). To measure the concentration of serum sIL-2r in dogs, an ELISA system was developed. Serum sIL-2r levels were significantly higher in dogs with multicentric high-grade B-cell lymphoma before therapy than that in healthy dogs. Serum sIL-2r concentration was also found to be elevated in a proportion of dogs with other types of lymphoma. Changes in serum sIL-2r levels generally paralleled the changes in mass and lymph node size in dogs with high-grade B-cell lymphoma. This study demonstrated that serum sIL-2r level would be a marker to monitor tumour growth and regression in canine lymphoma.

引用

页数：9

共 55 条

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