Characterization of water-soluble dark-brown pigment from Antarctic bacterium, Lysobacter oligotrophicus

被引：30

作者：

Kimura, Tomomi ^{[1
]}

Fukuda, Wakao ^{[1
]}

Sanada, Tomoe ^{[2
]}

Imanaka, Tadayuki ^{[1
]}

机构：

[1] Ritsumeikan Univ, Dept Biotechnol, Coll Life Sci, Kusatsu, Shiga 5258577, Japan

[2] Ritsumeikan Univ, Dept Appl Chem, Coll Life Sci, Kusatsu, Shiga 5258577, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 120卷 / 01期

关键词：

Antarctic; Melanin; Microbial product; Macromolecular structure; UV-protection; SP NOV; MELANIN PIGMENT; GEN; NOV; BIOSYNTHESIS; LAKE; MICROORGANISMS; MELANOGENESIS; PURIFICATION; RESONANCE;

D O I：

10.1016/j.jbiosc.2014.11.020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lysobacter oligotrophicus strain 107-E2(T) isolated from Antarctica produces dark-brown colored water-soluble pigment, in addition to hydrolases and lytic enzymes. The production of pigment is a common characteristic among members of the genus Lysobacter, but the identity of the pigments has been unknown. In this study, we identified the pigment from L oligotrophicus as melanin pigment (Lo-melanin) by chemical and spectroscopic analyses. Although melanin is generally insoluble in both aqueous and organic solvents, the results in this study revealed that Lo-melanin shows water-solubility by means of the added polysaccharide chain. Lo-melanin production of L. oligotrophicus was increased by ultraviolet (UV) exposure, and survival rate of Escherichia coli under UV-irradiated condition was increased by the addition of Lo-melanin to the medium. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：58 / 61

页数：4

共 41 条

[1] Melanogenesis: a photoprotective response to DNA damage? [J].

Agar, N ;

Young, AR .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2005, 571 (1-2) :121-132

[2] Obtaining of water soluble microbial melanin and study of its some properties [J].

Aghajanyan, A. A. ;

Asaturian, R. A. ;

Hambardzumyan, A. A. ;

Sargsyan, L. B. ;

Hovsepyan, A. S. ;

Vardanyan, A. H. ;

Saghyan, A. S. .

APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2011, 47 (05) :500-506

[3] Isolation, purification and physicochemical characterization of water-soluble Bacillus thuringiensis melanin [J].

Aghajanyan, AE ;

Hambardzumyan, AA ;

Hovsepyan, AS ;

Asaturian, RA ;

Vardanyan, AA ;

Saghiyan, AA .

PIGMENT CELL RESEARCH, 2005, 18 (02) :130-135

[4] Marine sponge melanin: a new source of an old biopolymer [J].

Araujo, Marco ;

Xavier, Joana R. ;

Nunes, Carla D. ;

Vaz, Pedro D. ;

Humanes, Madalena .

STRUCTURAL CHEMISTRY, 2012, 23 (01) :115-122

[5] BIOSYNTHESIS AND FUNCTIONS OF FUNGAL MELANINS [J].

BELL, AA ;

WHEELER, MH .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 1986, 24 :411-451

[6] ELECTRON SPIN RESONANCE STUDIES ON MELANIN [J].

BLOIS, MS ;

MALING, JE ;

ZAHLAN, AB .

BIOPHYSICAL JOURNAL, 1964, 4 (06) :471-&

[7]

Cabiscol Elisa, 2000, International Microbiology, V3, P3

[8] On the rocks: the microbiology of Antarctic Dry Valley soils [J].

Cary, S. Craig ;

McDonald, Ian R. ;

Barrett, John E. ;

Cowan, Don A. .

NATURE REVIEWS MICROBIOLOGY, 2010, 8 (02) :129-138

[9] Melanin and virulence in Cryptococcus neoformans [J].

Casadevall, A ;

Rosas, AL ;

Nosanchuk, JD .

CURRENT OPINION IN MICROBIOLOGY, 2000, 3 (04) :354-358

[10]

Christensen P :., 2005, Bergey's Manual of Systematic Bacteriology, V2, P95

← 1 2 3 4 5 →