TLR4 and S1P receptors cooperate to enhance inflammatory cytokine production in human gingival epithelial cells

被引:61
作者
Eskan, Mehmet A. [1 ]
Rose, Beate G. [1 ]
Benakanakere, Manjunatha R. [1 ]
Zeng, Qun [2 ]
Fujioka, Daisuke [1 ]
Martin, Michael H. [1 ]
Lee, Menq-Jer [2 ]
Kinane, Denis F. [1 ]
机构
[1] Univ Louisville, Sch Dent, Oral Hlth & Syst Dis, Louisville, KY 40202 USA
[2] Univ Louisville, Gheens Ctr Aging Microbiol & Immunol, Louisville, KY USA
关键词
cytokines; epithelial cells; S1P(1); S1P(3); TLR4;
D O I
10.1002/eji.200737898
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Toll-like receptors (TLR) are pattern recognition receptors for highly conserved microbial molecular patterns. Activation of TLR is a pivotal step in the initiation of innate, inflammatory, and immune defense mechanisms. Recent findings indicate that G protein-coupled receptors (GPCR) may modulate TLR signaling, but it is unclear which GPCR are involved in this process. One such cooperation between GPCR and TLR can be attributed to the sphingosine 1-phosphate (S1P) receptor family. The S1P receptors (S1P(1-5)) are a family of GPCR with a high affinity for S1P, a serum-borne bioactive lipid associated with diverse biological activities such as inflammation and healing. In this study, we show that pro-inflammatory cytokine production, including IL-6 and IL-8, was increased with LPS and concomitant S1P stimulation. Furthermore, elevated cytokine production following LPS and S1P challenge in human gingival epithelial cells (HGEC) was significantly reduced when TLR4, S1P(1) or S1P(3) signaling was blocked. Our study also shows that S1P(1) and S1P(3) expression was induced by LPS in HGEC, and this elevated expression enhanced the influence of S1P in its cooperation with TLR4 to increase cytokine production. This cooperation between TLR4 and S1P(1) or S1P3 demonstrates that TLR4 and GPCR can interact to enhance cytokine production in epithelial cells.
引用
收藏
页码:1138 / 1147
页数:10
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