A Brucella Omp16 Conditional Deletion Strain Is Attenuated in BALB/c Mice

被引:2
作者
Zhi, Feijie [1 ]
Fang, Jiaoyang [1 ]
Zheng, Weifang [1 ]
Li, Junmei [1 ]
Zhang, Guangdong [1 ]
Zhou, Dong [1 ,2 ]
Jin, Yaping [1 ,2 ]
Wang, Aihua [1 ,2 ]
机构
[1] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
[2] Northwest A&F Univ, Minist Agr, Key Lab Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
Brucella suis S2; Omp16; virulence; immune response; mice; OUTER-MEMBRANE PROPERTIES; IMMUNE-RESPONSE; CONFERS PROTECTION; VIRULENCE FACTORS; HUMORAL IMMUNITY; ABORTUS; MELITENSIS; INFECTION; PATHOGENESIS; OMP25/OMP31;
D O I
10.4014/jmb.2107.07016
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Brucella spp. are facultative intracellular pathogens that invade, survive and proliferate in numerous phagocytic and non-phagocytic cell types, thereby leading to human and animal brucellosis. Outer membrane proteins (Omps) are major immunogenic and protective antigens that are implicated in Brucella virulence. A strain deleted of the omp16 gene has not been obtained which suggests that the Omp16 protein is vital for Brucella survival. Nevertheless, we previously constructed an omp16 conditional deletion strain of Brucella, Delta Omp16. Here, the virulence and immune response elicted by this strain were assessed in a mouse model of infection. Splenomegaly was significantly reduced at two weeks post-infection in Delta Omp16-infected mice compared to infection with the parental strain. The bacterial load in the spleen also was significantly decreased at this post-infection time point in Delta Omp16-infected mice. Histopathological changes in the spleen were observed via hematoxylineosin staining and microscopic examination which showed that infection with the Delta Omp16 strain alleviated spleen histopathological alterations compared to mice infected with the parental strain. Moreover, the levels of humoral and cellular immunity were similar in both Delta Omp16-infected mice and parental strain-infected mice. The results overall show that the virulence of Delta Omp16 is attenuated markedly, but that the immune responses mediated by the deletion and parental strains in mice are indistinguishable. The data provide important insights that illuminate the pathogenic strategies adopted by Brucella.
引用
收藏
页码:6 / 14
页数:9
相关论文
共 47 条
[11]   Brucella species lacking the major outer membrane protein Omp25 are attenuated in mice and protect against Brucella melitensis and Brucella ovis [J].
Edmonds, MD ;
Cloeckaert, A ;
Elzer, PH .
VETERINARY MICROBIOLOGY, 2002, 88 (03) :205-221
[12]   Host response to Brucella infection: review and future perspective [J].
Elfaki, Mohamed G. ;
Alaidan, Alwaleed Abdullah ;
Al-Hokail, Abdullah Abdulrahman .
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 2015, 9 (07) :697-701
[13]   Rough brucella strain RM57 is attenuated and confers protection against Brucella melitensis [J].
Feng, Yu ;
Peng, Xiaowei ;
Jiang, Hui ;
Peng, Yong ;
Zhu, Liangquan ;
Ding, Jiabo .
MICROBIAL PATHOGENESIS, 2017, 107 :270-275
[14]   Brucella ceti sp nov and Brucella pinnipedialis sp nov for Brucella strains with cetaceans and seals as their preferred hosts [J].
Foster, Geoffrey ;
Osterman, Bjorn S. ;
Godfroid, Jacques ;
Jacques, Isabelle ;
Cloeckaert, Axel .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007, 57 :2688-2693
[15]   Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action [J].
Franc, K. A. ;
Krecek, R. C. ;
Haesler, B. N. ;
Arenas-Gamboa, A. M. .
BMC PUBLIC HEALTH, 2018, 18
[16]   Brucella - Virulence Factors, Pathogenesis and Treatment [J].
Glowacka, Patrycja ;
Zakowska, Dorota ;
Naylor, Katarzyna ;
Niemcewicz, Marcin ;
Bielawska-Drozd, Agata .
POLISH JOURNAL OF MICROBIOLOGY, 2018, 67 (02) :151-161
[17]   Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins [J].
Ibanez, Andres E. ;
Smaldini, Paola ;
Coria, Lorena M. ;
Delpino, Maria V. ;
Pacfico, Lucila G. G. ;
Oliveira, Sergio C. ;
Risso, Gabriela S. ;
Pasquevich, Karina A. ;
Alberto Fossati, Carlos ;
Giambartolomei, Guillermo H. ;
Docena, Guillermo H. ;
Cassataro, Juliana .
PLOS ONE, 2013, 8 (07)
[18]   Development and trial of vaccines against Brucella [J].
Lalsiamthara, Jonathan ;
Lee, John Hwa .
JOURNAL OF VETERINARY SCIENCE, 2017, 18 :281-290
[19]   Fun stories about Brucella:: the "furtive nasty bug" [J].
Letesson, JJ ;
Lestrate, P ;
Delrue, RM ;
Danese, I ;
Bellefontaine, F ;
Fretin, D ;
Taminiau, B ;
Tibor, A ;
Dricot, A ;
Deschamps, C ;
Haine, V ;
Leonard, S ;
Laurent, T ;
Mertens, P ;
Vandenhaute, J ;
De Bolle, X .
VETERINARY MICROBIOLOGY, 2002, 90 (1-4) :317-328
[20]   Construction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus [J].
Li, Tiansen ;
Huang, Meiling ;
Wang, Zhen ;
Guo, Fei ;
Zhang, Hui ;
Chen, Chuangfu .
PAKISTAN JOURNAL OF ZOOLOGY, 2017, 49 (05) :1809-1816