Role of microRNA221 in regulating normal mammary epithelial hierarchy and breast cancer stem-like cells

被引：44

作者：

Ke, Jia ^{[1
]}

Zhao, Zhiju ^{[2
,3
]}

Hong, Su-Hyung ^{[4
]}

Bai, Shoumin ^{[5
]}

He, Zhen ^{[1
]}

Malik, Fayaz ^{[6
]}

Xu, Jiahui ^{[2
,3
]}

Zhou, Lei ^{[2
,3
]}

Chen, Weilong ^{[2
,3
]}

Martin-Trevino, Rachel ^{[6
]}

Wu, Xiaojian ^{[1
]}

Lan, Ping ^{[1
]}

Yi, Yongju ^{[7
]}

Ginestier, Christophe ^{[8
]}

Ibarra, Ingrid ^{[9
]}

Shang, Li ^{[6
]}

McDermott, Sean ^{[6
]}

Luther, Tahra ^{[6
]}

Clouthier, Shawn G. ^{[6
]}

Wicha, Max S. ^{[6
]}

Liu, Suling ^{[2
,3
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Colorectal Surg, Guangzhou 510275, Guangdong, Peoples R China

[2] Univ Sci & Technol China, Innovat Ctr Cell Biol, Hefei 230026, Anhui, Peoples R China

[3] Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, Sch Life Sci & Med Ctr, Hefei 230026, Anhui, Peoples R China

[4] Kyungpook Natl Univ, Sch Dent, Dept Oral Microbiol, Daegu, South Korea

[5] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Oncol, Guangzhou 510275, Guangdong, Peoples R China

[6] Univ Michigan, Dept Internal Med, Comprehens Canc Ctr, Ann Arbor, MI 48109 USA

[7] Sun Yat Sen Univ, Affiliated Hosp 6, Network Informat Ctr, Guangzhou 510275, Guangdong, Peoples R China

[8] Univ Aix Marseille 2, Inst Paoli Calmettes, Inserm UMR891, Ctr Rech Cancerol Marseille,Lab Oncol Mol, Marseille, France

[9] Cold Spring Harbor Lab, Program Genet & Bioinformat, Cold Spring Harbor, NY 11724 USA

来源：

ONCOTARGET | 2015年 / 6卷 / 06期

关键词：

miR-221; breast stem-like cells; differentiation; hierarchy; ADULT HUMAN BREAST; IN-VITRO; BASAL; SUBPOPULATIONS; PREDICTOR; SURVIVAL; SUBTYPES; MIR-221; MARKER; LINES;

D O I：

10.18632/oncotarget.2888

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Increasing evidence suggests that lineage specific subpopulations and stem-like cells exist in normal and malignant breast tissues. Epigenetic mechanisms maintaining this hierarchical homeostasis remain to be investigated. In this study, we found the level of microRNA221 (miR-221) was higher in stem-like and myoepithelial cells than in luminal cells isolated from normal and malignant breast tissue. In normal breast cells, over-expression of miR-221 generated more myoepithelial cells whereas knockdown of miR-221 increased luminal cells. Over-expression of miR-221 stimulated stem-like cells in luminal type of cancer and the miR-221 level was correlated with clinical outcome in breast cancer patients. Epithelial-mesenchymal transition (EMT) was induced by overexpression of miR-221 in normal and breast cancer cells. The EMT related gene ATXN1 was found to be a miR-221 target gene regulating breast cell hierarchy. In conclusion, we propose that miR-221 contributes to lineage homeostasis of normal and malignant breast epithelium.

引用

页码：3709 / 3721

页数：13

共 32 条

[11] Ataxin-1 nuclear localization and aggregation:: Role in polyglutamine-induced disease in SCA1 transgenic mice [J].

Klement, IA ;

Skinner, PJ ;

Kaytor, MD ;

Yi, H ;

Hersch, SM ;

Clark, HB ;

Zoghbi, HY ;

Orr, HT .

CELL, 1998, 95 (01) :41-53

[12] Ataxin-1 occupies the promoter region of E-cadherin in vivo and activates CtBP2-repressed promoter [J].

Lee, Soyeon ;

Hong, Sunghoi ;

Kim, Sungsu ;

Kang, Seongman .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2011, 1813 (05) :713-722

[13] Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers [J].

Lim, Elgene ;

Vaillant, Francois ;

Wu, Di ;

Forrest, Natasha C. ;

Pal, Bhupinder ;

Hart, Adam H. ;

Asselin-Labat, Marie-Liesse ;

Gyorki, David E. ;

Ward, Teresa ;

Partanen, Audrey ;

Feleppa, Frank ;

Huschtscha, Lily I. ;

Thorne, Heather J. ;

Fox, Stephen B. ;

Yan, Max ;

French, Juliet D. ;

Brown, Melissa A. ;

Smyth, Gordon K. ;

Visvader, Jane E. ;

Lindeman, Geoffrey J. .

NATURE MEDICINE, 2009, 15 (08) :907-913

[14] BRCA1 regulates human mammary stem/progenitor cell fate [J].

Liu, Suling ;

Ginestier, Christophe ;

Charafe-Jauffret, Emmanuelle ;

Foco, Hailey ;

Kleer, Celina G. ;

Merajver, Sofia D. ;

Dontu, Gabriela ;

Wicha, Max S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (05) :1680-1685

[15] MicroRNA93 Regulates Proliferation and Differentiation of Normal and Malignant Breast Stem Cells [J].

Liu, Suling ;

Patel, Shivani H. ;

Ginestier, Christophe ;

Ibarra, Ingrid ;

Martin-Trevino, Rachel ;

Bai, Shoumin ;

McDermott, Sean P. ;

Shang, Li ;

Ke, Jia ;

Ou, Sing J. ;

Heath, Amber ;

Zhang, Kevin J. ;

Korkaya, Hasan ;

Clouthier, Shawn G. ;

Charafe-Jauffret, Emmanuelle ;

Birnbaum, Daniel ;

Hannon, Gregory J. ;

Wicha, Max S. .

PLOS GENETICS, 2012, 8 (06)

[16] Breast Cancer Stem Cells Are Regulated by Mesenchymal Stem Cells through Cytokine Networks [J].

Liu, Suling ;

Ginestier, Christophe ;

Ou, Sing J. ;

Clouthier, Shawn G. ;

Patel, Shivani H. ;

Monville, Florence ;

Korkaya, Hasan ;

Heath, Amber ;

Dutcher, Julie ;

Kleer, Celina G. ;

Jung, Younghun ;

Dontu, Gabriela ;

Taichman, Russell ;

Wicha, Max S. .

CANCER RESEARCH, 2011, 71 (02) :614-624

[17] MicroRNA-221/222 Confers Tamoxifen Resistance in Breast Cancer by Targeting p27Kip1 [J].

Miller, Tyler E. ;

Ghoshal, Kalpana ;

Ramaswamy, Bhuvaneswari ;

Roy, Satavisha ;

Datta, Jharna ;

Shapiro, Charles L. ;

Jacob, Samson ;

Majumder, Sarmila .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (44) :29897-29903

[18] Availability of CD10 immunohistochemistry as a marker of breast myoepithelial cells on paraffin sections [J].

Moritani, S ;

Kushima, R ;

Sugihara, H ;

Bamba, M ;

Kobayashi, TK ;

Hattori, T .

MODERN PATHOLOGY, 2002, 15 (04) :397-405

[19] A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes [J].

Neve, Richard M. ;

Chin, Koei ;

Fridlyand, Jane ;

Yeh, Jennifer ;

Baehner, Frederick L. ;

Fevr, Tea ;

Clark, Laura ;

Bayani, Nora ;

Coppe, Jean-Philippe ;

Tong, Frances ;

Speed, Terry ;

Spellman, Paul T. ;

DeVries, Sandy ;

Lapuk, Anna ;

Wang, Nick J. ;

Kuo, Wen-Lin ;

Stilwell, Jackie L. ;

Pinkel, Daniel ;

Albertson, Donna G. ;

Waldman, Frederic M. ;

McCormick, Frank ;

Dickson, Robert B. ;

Johnson, Michael D. ;

Lippman, Marc ;

Ethier, Stephen ;

Gazdar, Adi ;

Gray, Joe W. .

CANCER CELL, 2006, 10 (06) :515-527

[20] miR-221 Silencing Blocks Hepatocellular Carcinoma and Promotes Survival [J].

Park, Jong-Kook ;

Kogure, Takayuki ;

Nuovo, Gerard J. ;

Jiang, Jinmai ;

He, Lei ;

Kim, Ji Hye ;

Phelps, Mitch A. ;

Papenfuss, Tracey L. ;

Croce, Carlo M. ;

Patel, Tushar ;

Schmittgen, Thomas D. .

CANCER RESEARCH, 2011, 71 (24) :7608-7616

← 1 2 3 4 →