Chemosensing of honeybee parasite, Varroa destructor: Transcriptomic analysis

被引：28

作者：

Eliash, Nurit ^{[1
,2
]}

Singh, Nitin K. ^{[1
]}

Thangarajan, Starlin ^{[1
]}

Sela, Noa ^{[1
]}

Leshkowitz, Dena ^{[3
]}

Kamer, Yosi ^{[1
]}

Zaidman, Ilia ^{[1
]}

Rafaeli, Ada ^{[4
]}

Soroker, Victoria ^{[1
]}

机构：

[1] Agr Res Org, Inst Plant Protect, Volcani Ctr, Rishon Leziyyon, Israel

[2] Hebrew Univ Jerusalem, Inst Agroecol & Plant Hlth, Robert H Smith Fac Agr Food & Environm, Rehovot, Israel

[3] Weizmann Inst Sci, Dept Biol Serv, Rehovot, Israel

[4] Agr Res Org, Inst Postharvest & Food Sci, Dept Food Qual & Safety, Volcani Ctr, Rishon Leziyyon, Israel

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

以色列科学基金会;

关键词：

IONOTROPIC GLUTAMATE RECEPTORS; CHEMICAL COMMUNICATION; ANTENNAL TRANSCRIPTOME; ODORANT RECEPTION; SOLUBLE-PROTEINS; ACARI IXODIDAE; HOST; GENES; BINDING; IDENTIFICATION;

D O I：

10.1038/s41598-017-13167-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chemosensing is a primary sense in nature, however little is known about its mechanism in Chelicerata. As a model organism we used the mite Varroa destructor, a key parasite of honeybees. Here we describe a transcriptomic analysis of two physiological stages for the Varroa foreleg, the site of primary olfactory organ. The transcriptomic analysis revealed transcripts of chemosensory related genes belonging to several groups. These include Niemann-Pick disease protein, type C2 (NPC2), gustatory receptors (GRs), ionotropic receptors (IRs), sensory neuron membrane proteins (SNMPs) and odorant binding proteins (OBP). However, no insect odorant receptors (ORs) and odorant co-receptors (ORcos) were found. In addition, we identified a homolog of the most ancient IR co-receptor, IR25a, in Varroa as well as in other members of Acari. High expression of this transcript in the mite's forelegs, while not detectable in the other pairs of legs, suggests a function for this IR25a-like in Varroa chemosensing.

引用

页数：11

共 58 条

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