Colorimetric assay of tyrosinase inhibition using melanocyte laden hydrogel fabricated by digital light processing printing

被引：4
作者：
Kwak, Hojung [1 ]
Shin, Sungchul [1 ]
Hyun, Jinho [1 ,2 ]
机构：
[1] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South Korea
[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea
来源：
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | 2020年 / 84卷
关键词：
Colorimetric assay; Melanocyte; Cell-laden hydrogel; Tyrosine inhibitors; Digital light processing; 3D printing; Polyethylene glycol tetraacrylate; KOJIC ACID; DUAL-EMISSION; IN-VITRO; NANOPARTICLES; DOPAMINE; BIOLOGY; DOTS;
D O I：
10.1016/j.jiec.2020.01.006
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Colorimetric assay of melanogenesis inhibition was demonstrated with polyethylene glycol tetraacrylate (PEG-4A) hydrogel structures embedding melanocyte cells. The cell laden PEG-4A hydrogel structure was prepared by digital light processing printing using biocompatible visible range of light. The melanocyte in PEG-4A hydrogel structure showed a high cell compatibility and expressed melanin changing a color of hydrogel to black. The inhibition of melanin expression was evaluated by injecting a different concentration of arbutin and kojic acid to the cell laden hydrogel structure in a 3D printed microfluidic chip. The tyrosinase inhibitors prevented a color change of the hydrogel pad according to the dosage showing a similar detecting efficiency to a commercial melanogenesis assay kit. (C) 2020 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：252 / 259
页数：8
相关论文
共 42 条
[1]   A fluorometric biosensor based on functional Au/Ag nanoclusters for real-time monitoring of tyrosinase activity [J].
Ao, Hang ;
Qian, Zhaosheng ;
Zhu, Yuyu ;
Zhao, Meizhi ;
Tang, Cong ;
Huang, Yuanyuan ;
Feng, Hui ;
Wang, Aijun .
BIOSENSORS & BIOELECTRONICS, 2016, 86 :542-547
[2]   KOJIC ACID, A COSMETIC SKIN WHITENING AGENT, IS A SLOW-BINDING INHIBITOR OF CATECHOLASE ACTIVITY OF TYROSINASE [J].
CABANES, J ;
CHAZARRA, S ;
GARCIACARMONA, F .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 1994, 46 (12) :982-985
[3]   Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation [J].
Chai, Lujing ;
Zhou, Jin ;
Feng, Hui ;
Tang, Cong ;
Huang, Yuanyuan ;
Qian, Zhaosheng .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (42) :23564-23574
[4]   Quantitative analysis of melanin content in a three-dimensional melanoma cell culture [J].
Chung, Soobin ;
Lim, Gippeum J. ;
Lee, JiYoun .
SCIENTIFIC REPORTS, 2019, 9 (1)
[5]   Wearable Wireless Tyrosinase Bandage and Microneedle Sensors: Toward Melanoma Screening [J].
Ciui, Bianca ;
Martin, Aida ;
Mishra, Rupesh K. ;
Brunetti, Barbara ;
Nakagawa, Tatsuo ;
Dawkins, Thomas J. ;
Lyu, Mengjia ;
Cristea, Cecilia ;
Sandulescu, Robert ;
Wang, Joseph .
ADVANCED HEALTHCARE MATERIALS, 2018, 7 (07)
[6]   Thiol-Epoxy "Click" Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs [J].
Cong Truc Huynh ;
Liu, Fangze ;
Cheng, Yuxuan ;
Coughlin, Katherine A. ;
Alsberg, Eben .
ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (31) :25936-25942
[7]   UV Radiation and the Skin [J].
D'Orazio, John ;
Jarrett, Stuart ;
Amaro-Ortiz, Alexandra ;
Scott, Timothy .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (06) :12222-12248
[8]  
Dan-Ning H., 2008, PHOTOCHEM PHOTOBIOL, V84, P645
[9]   Colorimetric tyrosinase assay based on catechol inhibition of the oxidase-mimicking activity of chitosan-stabilized platinum nanoparticles [J].
Deng, Hao-Hua ;
Lin, Xiu-Ling ;
He, Shao-Bin ;
Wu, Gang-Wei ;
Wu, Wei-Hua ;
Yang, Yu ;
Lin, Zhen ;
Peng, Hua-Ping ;
Xia, Xing-Hua ;
Chen, Wei .
MICROCHIMICA ACTA, 2019, 186 (05)
[10]   Reactions of Flavonoids with o-Quinones Interfere with the Spectrophotometric Assay of Tyrosinase Activity [J].
Gasowska-Bajger, Beata ;
Wojtasek, Hubert .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (26) :5417-5427
12345