Adaptive optics wide-field microscopy using direct wavefront sensing

被引：86

作者：

Azucena, Oscar ^{[1
]}

Crest, Justin

Kotadia, Shaila

Sullivan, William

Tao, Xiaodong ^{[1
]}

Reinig, Marc ^{[2
]}

Gavel, Donald ^{[2
]}

Olivier, Scot ^{[3
]}

Kubby, Joel ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Jack Baskin Sch Engn, Santa Cruz, CA 95064 USA

[2] Univ Calif Santa Cruz, Lab Adapt Opt, Santa Cruz, CA 95064 USA

[3] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

来源：

OPTICS LETTERS | 2011年 / 36卷 / 06期

基金：

美国国家卫生研究院;

关键词：

HUMAN EYE; ABERRATIONS; SCATTERING;

D O I：

10.1364/OL.36.000825

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report a technique for measuring and correcting the wavefront aberrations introduced by a biological sample using a Shack-Hartmann wavefront sensor, a fluorescent reference source, and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved. (C) 2011 Optical Society of America

引用

页码：825 / 827

页数：3

共 15 条

[1] Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction [J].

Azucena, Oscar ;

Crest, Justin ;

Cao, Jian ;

Sullivan, William ;

Kner, Peter ;

Gavel, Donald ;

Dillon, Daren ;

Olivier, Scot ;

Kubby, Joel .

MEMS ADAPTIVE OPTICS IV, 2010, 7595

[2] Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons [J].

Azucena, Oscar ;

Crest, Justin ;

Cao, Jian ;

Sullivan, William ;

Kner, Peter ;

Gavel, Donald ;

Dillon, Daren ;

Olivier, Scot ;

Kubby, Joel .

OPTICS EXPRESS, 2010, 18 (16) :17521-17532

[3] Implementation of a Shack-Hartmann Wavefront Sensor for the measurement of embryo induced aberrations using fluorescent microscopy [J].

Azucena, Oscar ;

Kubby, Joel ;

Crest, Justin ;

Cao, Jian ;

Sullivan, William ;

Kner, Peter ;

Gavel, Donald ;

Dillon, Daren ;

Olivier, Scot .

MEMS ADAPTIVE OPTICS III, 2009, 7209

[4] Adaptive optics in microscopy [J].

Booth, Martin J. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2007, 365 (1861) :2829-2843

[5]

Diaz Santana Haro L, 1999, Opt Lett, V24, P61

[6] Three-dimensional computation of light scattering from cells [J].

Dunn, A ;

RichardsKortum, R .

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 1996, 2 (04) :898-905

[7] Coherence-gated wave-front sensing in strongly scattering samples [J].

Feierabend, M ;

Rückel, M ;

Denk, W .

OPTICS LETTERS, 2004, 29 (19) :2255-2257

[8] Suppressing anomalous localized waffle behavior in least squares wavefront reconstructors [J].

Gavel, DT .

ADAPTIVE OPTICAL SYSTEM TECHNOLOGIES II, PTS 1 AND 2, 2003, 4839 :972-980

[9]

Hardy J. W., 1998, ADAPTIVE OPTICS ASTR

[10] OBJECTIVE MEASUREMENT OF WAVE ABERRATIONS OF THE HUMAN EYE WITH THE USE OF A HARTMANN-SHACK WAVE-FRONT SENSOR [J].

LIANG, JZ ;

GRIMM, B ;

GOELZ, S ;

BILLE, JF .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1994, 11 (07) :1949-1957

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