New trimethoprim-resistant dihydrofolate reductase cassette, dfrXV, inserted in a class 1 integron

被引：18
作者：
Adrian, PV
du Plessis, M
Klugman, KP
Amyes, SGB
机构：
[1] S African Inst Med Res, Dept Med Microbiol, ZA-2000 Johannesburg, South Africa
[2] Univ Witwatersrand, Dept Med Microbiol, Johannesburg, South Africa
[3] Univ Edinburgh, Dept Med Microbiol, Edinburgh, Midlothian, Scotland
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1998年 / 42卷 / 09期
关键词：
D O I：
10.1128/AAC.42.9.2221
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV, which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ant (3 ")-Ia], and sulfonamide resistance, sul1, were located downstream of dfrXV in a manner identical to that in pLMO229.
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页码：2221 / 2224
页数：4
相关论文
共 31 条
[11]   THE REGION OF THE INCN PLASMID R46 CODING FOR RESISTANCE TO BETA-LACTAM ANTIBIOTICS, STREPTOMYCIN-SPECTINOMYCIN AND SULFONAMIDES IS CLOSELY RELATED TO ANTIBIOTIC-RESISTANCE SEGMENTS FOUND IN INCW PLASMIDS AND IN TN21-LIKE TRANSPOSONS [J].
HALL, RM ;
VOCKLER, C .
NUCLEIC ACIDS RESEARCH, 1987, 15 (18) :7491-7501
[12]   NUCLEOTIDE-SEQUENCE ANALYSIS OF A GENE ENCODING A STREPTOMYCIN SPECTINOMYCIN ADENYLTRANSFERASE [J].
HOLLINGSHEAD, S ;
VAPNEK, D .
PLASMID, 1985, 13 (01) :17-30
[13]   TRIMETHOPRIM AND SULFONAMIDE RESISTANCE [J].
HUOVINEN, P ;
SUNDSTROM, L ;
SWEDBERG, G ;
SKOLD, O .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (02) :279-289
[14]   DIVERSITY AND RELATIVE STRENGTH OF TANDEM PROMOTERS FOR THE ANTIBIOTIC-RESISTANCE GENES OF SEVERAL INTEGRONS [J].
LEVESQUE, C ;
BRASSARD, S ;
LAPOINTE, J ;
ROY, PH .
GENE, 1994, 142 (01) :49-54
[15]   TRANSPOSON TN21 ENCODES A RECA-INDEPENDENT SITE-SPECIFIC INTEGRATION SYSTEM [J].
MARTINEZ, E ;
DELACRUZ, F .
MOLECULAR & GENERAL GENETICS, 1988, 211 (02) :320-325
[16]  
NOVAK P, 1983, J BIOL CHEM, V258, P956
[17]  
OSBORN MJ, 1958, J BIOL CHEM, V233, P969
[18]   HOMOLOGY OF ORFS FROM TN2603 AND FROM R46 TO SITE-SPECIFIC RECOMBINASES [J].
OUELLETTE, M ;
ROY, PH .
NUCLEIC ACIDS RESEARCH, 1987, 15 (23) :10055-10055
[19]  
PATTISHALL KH, 1977, J BIOL CHEM, V252, P2319
[20]   TRANSPOSON TN5090 OF PLASMID R751, WHICH CARRIES AN INTEGRON, IS RELATED TO TN7, MU, AND THE RETROELEMENTS [J].
RADSTROM, P ;
SKOLD, O ;
SWEDBERG, G ;
FLENSBURG, J ;
ROY, PH ;
SUNDSTROM, L .
JOURNAL OF BACTERIOLOGY, 1994, 176 (11) :3257-3268
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