Decreased expression of CD69 in chronic fatigue syndrome in relation to inflammatory markers: evidence for a severe disorder in the early activation of T Lymphocytes and natural killer cells

被引:0
作者
Mihaylova, Ivana
Deruyter, Marcel
Rummens, Jean-Luc
Bosmans, Eugene
Maes, Michael
机构
[1] MCare4U Outpatient Clin, B-2610 Antwerp, Belgium
[2] Salvator Hosp, Lab Clin Biochem, Hasselt, Belgium
[3] Virga Jesse Hosp, Lab Clin Biochem, Hasselt, Belgium
[4] AML Lab, Antwerp, Belgium
[5] Vanderbilt Univ, Dept Psychiat, Nashville, TN USA
关键词
chronic fatigue syndrome; cytokines; immunity; T lymphocytes; CD69; immune activation; natural killer cells; PKC; PKT;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
There is some evidence that patients with chronic fatigue syndrome (CFS) suffer from immune abnormalities, such as immune activation and decreased immune cell responsivity upon polyclonal stimili. This study was designed to evaluate lymphocyte activation in CFS by using a CD69 cxpression assay. CD69 acts as a costimulatory molecule for T- and natural killer (NK) cell activation. We collected whole blood from CFS patients, who met CDC criteria, and healthy volunteers. The blood samples were stimulated with mitogens during 18 It and the levels of activated T and NK cells expressing CD69 were measured on a Coulter Epics flow cytometer using a three color immunofluorescence staining protocol. The expression of the CD69 activation marker on T cells (CD3+, CD3+CD4+, and CD3+CD8+) and on NK cells (CD45+CD56+) was significantly lower in CFS patients than in healthy subjects. These differences were significant to the extent that a significant diagnostic performance was obtained, i.e. the area under the ROC curve was around 89%. No differences either in the number of leukocytes or in the number or percentage of lymphocytes, i.e. CD3, CD4, CD8 and CD19, could be found between CFS patients and the controls. Patients with CFS show defects in T- and NK cell activation. Since induction of CD69 surface expression is dependent on the activation of the protein kinase C (PKC) activation pathway, it is suggested that in CFS there is a disorder in the early activation of the immune system involving PKC.
引用
收藏
页码:477 / 483
页数:7
相关论文
共 44 条
  • [1] Altman A, 1990, Adv Immunol, V48, P227, DOI 10.1016/S0065-2776(08)60756-7
  • [2] LOW NK SYNDROME AND ITS RELATIONSHIP TO CHRONIC FATIGUE SYNDROME
    AOKI, T
    MIYAKOSHI, H
    USUDA, Y
    HERBERMAN, RB
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1993, 69 (03): : 253 - 265
  • [3] IMMUNOLOGICAL ABNORMALITIES ASSOCIATED WITH CHRONIC FATIGUE SYNDROME
    BARKER, E
    FUJIMURA, SF
    FADEM, MB
    LANDAY, AL
    LEVY, JA
    [J]. CLINICAL INFECTIOUS DISEASES, 1994, 18 : S136 - S141
  • [4] CD69 is a stimulatory receptor for natural killer cell and its cytotoxic effect is blocked by CD94 inhibitory receptor
    Borrego, F
    Robertson, MJ
    Ritz, J
    Peña, J
    Solana, R
    [J]. IMMUNOLOGY, 1999, 97 (01) : 159 - 165
  • [5] REGULATION OF CD69 EXPRESSION ON HUMAN NATURAL-KILLER-CELLS - DIFFERENTIAL INVOLVEMENT OF PROTEIN-KINASE-C AND PROTEIN-TYROSINE KINASES
    BORREGO, F
    PENA, J
    SOLANA, R
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (05) : 1039 - 1043
  • [6] BUCHWALD D, 1991, REV INFECT DIS, V13, pS12
  • [7] CALIGIURI M, 1987, J IMMUNOL, V139, P3306
  • [8] Caruso A, 1997, CYTOMETRY, V27, P71, DOI 10.1002/(SICI)1097-0320(19970101)27:1<71::AID-CYTO9>3.0.CO
  • [9] 2-O
  • [10] EXPRESSION AND FUNCTION OF AIM, AN ACTIVATION INDUCER MOLECULE OF HUMAN-LYMPHOCYTES, IS DEPENDENT ON THE ACTIVATION OF PROTEIN KINASE-C
    CEBRIAN, M
    REDONDO, JM
    LOPEZRIVAS, A
    RODRIGUEZTARDUCHY, G
    DELANDAZURI, MO
    SANCHEZMADRID, F
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (05) : 809 - 815