Rapid and efficient DNA strand cross-linking by click chemistry

被引：69
作者：
Kocalka, Petr [1 ]
El-Sagheer, Afaf H. [1 ]
Brown, Tom [1 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
来源：
CHEMBIOCHEM | 2008年 / 9卷 / 08期
关键词：
click chemistry; cross-linking; CuAAC reaction; DNA;
D O I：
10.1002/cbic.200800006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Click chemistry has been used to covolently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.
引用
收藏
页码：1280 / 1285
页数：6
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