Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface

被引：22
作者：
Van Berkel, Gary J. [1 ]
Kertesz, Vilmos [1 ]
Boeltz, Harry [2 ]
机构：
[1] Oak Ridge Natl Lab, Chem Sci Div, Mass Spectrometry & Laser Spect Grp, Oak Ridge, TN 37831 USA
[2] Dispendix GmbH, Meitnerstr 9, D-70563 Stuttgart, Germany
来源：
BIOANALYSIS | 2017年 / 9卷 / 21期
基金：
美国能源部;
关键词：
drop dispensing; electrospray ionization; immediate drop on demand; mass spectrometry; microtiter plates; noncontact dispensing; open port sampling interface; THROUGHPUT; ABLATION; PROBE; WATER; MS;
D O I：
10.4155/bio-2017-0104
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: The aim of this work was to demonstrate and evaluate the analytical performance of coupling the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. Methodology & results: A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. Conclusion: This immediate drop on demand technology/open port sampling interface/ESI-MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.
引用
收藏
页码：1667 / 1679
页数：13
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