Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

被引：24

作者：

Ahuka-Mundeke, Steve ^{[1
,2
,3
]}

Ayouba, Ahidjo ^{[1
]}

Mbala-Kingebeni, Placide ^{[2
,3
]}

Liegeois, Florian ^{[1
]}

Esteban, Amandine ^{[1
]}

Lunguya-Metila, Octavie ^{[2
,3
]}

Demba, Didace

Bilulu, Guy

Mbenzo-Abokome, Valentin ^{[4
]}

Inogwabini, Bila-Isia ^{[4
]}

Muyembe-Tamfum, Jean-Jacques ^{[2
,3
]}

Delaporte, Eric ^{[1
]}

Peeters, Martine ^{[1
]}

机构：

[1] Univ Montpellier, F-34059 Montpellier, France

[2] Inst Natl Rech Biomed, Kinshasa, DEM REP CONGO

[3] Clin Univ Kinshasa, Kinshasa, DEM REP CONGO

[4] World Wildlife Fund Nat, Kinshasa, DEM REP CONGO

来源：

EMERGING INFECTIOUS DISEASES | 2011年 / 17卷 / 12期

关键词：

NONHUMAN-PRIMATES; GENETIC DIVERSITY; INFECTION; EMERGENCE; EPIDEMIC; WILD; TRANSMISSION; LENTIVIRUS; SEQUENCE; HEALTH;

D O I：

10.3201/eid1712.110783

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Like most emerging infectious disease viruses, HIV is also of Zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of the Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.

引用

页码：2277 / 2286

页数：10

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