Changes in composition of grape seed oil during vegetation

This study was done to show what changes occur in the composition of developing seeds of Bulgarian grape variety (Super ran bolgar). The investigation was carried out on the, 50-th, 80-th and 110-th day after flowering (DAF). The grape sample collected at an early stage (50-th) showed very low oil content (0.7%) compared with the grapes collected later (13.9% on the 80-th and 16.5% on the 1104 DAF). The content of proteins decreased from 11.7% to 6.3% and of carbohydrates from 70.2% to 68.2% during different stages of berry development. Other biologically active components such as phospholipids, sterols and tocopherols are formed almost completely in the first stage during vegetation. In the third stage of the picking (110 day of growing) their quantity decreased significantly with the exception of the amount of sterols from 0.2% on the 80-th day to 0.3% on the 110-th day. The content of phospholipids dropped from 24.2% to 0.6% and that of tocopherols from 1727.9 mg/kg in the first stage of development to 67.7 mg/kg in the last stage, respectively. In the triacylglycerol fraction during the whole period of vegetation was found that the content of the essential linoleic acid increased from 49.8% to 72.3%, oleic from 15.9% to 17.3% and palmitic decreased from 24.1% to 8.8%.