West Nile virus-induced bax-dependent apoptosis

被引：97
作者：
Parquet, MD
Kumatori, A
Hasebe, F
Morita, K
Igarashi, A
机构：
[1] Nagasaki Univ, Inst Trop Med, Dept Virol, Nagasaki 8528523, Japan
[2] Nagasaki Univ, Inst Trop Med, Dept Biochem, Nagasaki 8528523, Japan
来源：
FEBS LETTERS | 2001年 / 500卷 / 1-2期
关键词：
flavivirus; West Nile virus; apoptosis; cell death; bcl-2; bax;
D O I：
10.1016/S0014-5793(01)02573-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mechanism of cell death induced by West Nile virus (WNV), a causative agent of human febrile syndrome and encephalitis, mas investigated. WNV-infected K562 and Neuro-2a cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and subdiploid DNA content by flow cytometry, DNA fragmentation into nucleosomal size and changes in outer cell membrane phospholipid composition mere also observed in K562 cells. UV-inactivated virus failed to induce the above-mentioned characteristics, suggesting that viral replication map be required for the induction of apoptosis by WNV, Additionally, signals involved in WNV-induced apoptosis are associated with the up-regulation of bax gene expression. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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收藏
页码：17 / 24
页数：8
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