Production of Polyhydroxyalkanoate Copolymers in Transgenic Plants Expressing Engineered Enzymes

被引：0
作者：
Matsumoto, Ken'ichiro [1 ]
机构：
[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan
来源：
KOBUNSHI RONBUNSHU | 2011年 / 68卷 / 08期
关键词：
Polyhydroxyalkanoate; PHA; PHA Synthase; Plant Factory; Engineered Enzyme; Biomass; Bio-Based Plastic; PSEUDOMONAS-SP; 61-3; RECOMBINANT ESCHERICHIA-COLI; PROTEIN SYNTHASE-III; PHA SYNTHASE; ARABIDOPSIS-THALIANA; AEROMONAS-CAVIAE; BETA-OXIDATION; POLYHYDROXYBUTYRATE; BIOSYNTHESIS; GENE;
D O I：
10.1295/koron.68.562
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Polyhydroxyalkanoates (PHAs) are bacterial polyesters that can be an alternative to petroleum-derived plastics. Transgenic plants harboring PHA biosynthetic genes are capable of producing PHAs directly from atmospheric CO2. The properties of PHAs greatly depend on their monomer composition. Therefore, fine regulation of monomer composition is essential to the production of PHAs with the desired properties. In order to synthesize PHA copolymer in plants, the supply of monomers and the expression of PHA synthase (polymerizing enzyme) having a broad polymerizing capacity are required. We found that the engineered enzymes with enhanced activity and/or altered substrate specificity are functional in plant cells. This finding enabled us to produce PHA copolymer in transgenic plants.
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页码：562 / 569
页数：8
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