Determination of the parental pronuclear origin in bovine zygotes: H3K9me3 versus H3K27me2-3

被引：3

作者：

Heras, Sonia ^{[1
]}

Vandenberghe, Lynn ^{[1
]}

Van Soom, Ann ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, B-9820 Merelbeke, Belgium

来源：

ANALYTICAL BIOCHEMISTRY | 2016年 / 510卷

关键词：

Immunofluorescence; Epigenetics; H3K9me3; H3K27me2-3; Pronuclear sexing; Bovine zygotes; DNA METHYLATION; MAMMALIAN DEVELOPMENT; 5-HYDROXYMETHYLCYTOSINE; MOUSE; DEMETHYLATION; CHROMATIN;

D O I：

10.1016/j.ab.2016.07.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the dynamics of 5-methylcytosine and 5-hydroxymethylcytosine in zygotes, the parental origin of the pronuclei needs to be determined. To this end the use of the asymmetric distribution of histone modifications in pronuclei is becoming more popular. Here, we demonstrated that histone 3 lysine 27 di-tri-methylation shows a stable pattern being present in the maternal but not in the paternal pronucleus of bovine zygotes, even in late stages of pronuclear development. In contrast, the pattern of histone 3 lysine 9 tri-methylation is very variable, and therefore cannot be used to reliably determine the parental origin of bovine pronuclei. (C) 2016 Elsevier Inc. All rights reserved.

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页码：76 / 78

页数：3

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