Multiple dose vaccination with heterologous H5N2 vaccine: Immune response and protection against variant clade 2.2.1 highly pathogenic avian influenza H5N1 in broiler breeder chickens

被引:36
作者
Abdelwhab, E. M. [1 ,3 ]
Grund, Christian [2 ]
Aly, Mona M. [3 ]
Beer, Martin [2 ]
Harder, Timm C. [2 ]
Hafez, Hafez M. [1 ]
机构
[1] Free Univ Berlin, Inst Poultry Dis, D-14163 Berlin, Germany
[2] Friedrich Loeffler Inst, D-17493 Greifswald, Germany
[3] Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod NLQP, Giza 12618, Egypt
关键词
Highly pathogenic avian influenza; H5N1; Antigenic drift; Vaccination; Egypt; Broiler breeders; Vertical; Egg-borne infection; NEWCASTLE-DISEASE; VIRUS H5N1; A VIRUSES; EGGS; HEMAGGLUTININ;
D O I
10.1016/j.vaccine.2011.06.090
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Circulation of an antigenically variant lineage of highly pathogenic avian influenza (HPAI) H5N1 virus in chicken breeder flocks in Egypt is a continuing problem. The protective efficacy of multiple repeated vaccinations using the currently available H5N2 vaccines is unclear. Here, broiler breeder chickens were vaccinated at weeks 6, 12 and 18 with an inactivated H5N2 commercial vaccine. HI-titer against an Egyptian H5N1 field isolate of classic clade 2.2.1 (EGYc1s/H5N1) were significantly lower after the first immunization but increased after booster vaccinations. In contrast, no HI titers were induced against an antigenically distinct field virus of the variant lineage of clade 2.2.1 (EGYvar/H5N1). Upon challenge at week 50 mild, if any, clinical signs were observed in the group infected with EGYcls/H5N1 although one of eight (12.5%) birds died. Mortality reached 6/8 (75%) in the EGYvar/H5N1 challenge group. Virus excretion in all vaccinated groups was reduced in amplitude, but in vaccinated surviving birds, time of virus excretion was extended to up to ten days. Strikingly, challenged vaccinated birds kept laying eggs almost throughout the observation period. Virus was detected on the outer egg-shell of 17 of 40 eggs. The majority of the infected eggs were derived from the EGYc1s/H5N1 challenged animals: here the virus was detected also in the yolk and albumin. Repeated vaccination using a commercial H5N2-based vaccine broadened the antigen profile of induced antibodies but did not provide adequate protection against heterologous virus variant. In addition, the observation of AIV contaminated eggs from infected flocks highlights the risk of silent virus spread by vaccinated animals and point to eggs as a possible vector. (C) 2011 Published by Elsevier Ltd.
引用
收藏
页码:6219 / 6225
页数:7
相关论文
共 37 条
[1]  
Abdelwhab EM, 2011, EPIDEMIOL INFECT
[2]   Epidemiological Findings of Outbreaks of Disease Caused by Highly Pathogenic H5N1 Avian Influenza Virus in Poultry in Egypt During 2006 [J].
Aly, M. M. ;
Arafa, A. ;
Hassan, M. K. .
AVIAN DISEASES, 2008, 52 (02) :269-277
[3]   Phylogenetic Analysis of Hemagglutinin and Neuraminidase Genes of Highly Pathogenic Avian Influenza H5N1 Egyptian Strains Isolated from 2006 to 2008 Indicates Heterogeneity with Multiple Distinct Sublineages [J].
Arafa, A. ;
Suarez, D. L. ;
Hassan, M. K. ;
Aly, M. M. .
AVIAN DISEASES, 2010, 54 (01) :345-349
[4]   Antigenic and Genetic Diversity of Highly Pathogenic Avian Influenza A (H5N1) Viruses Isolated in Egypt [J].
Balish, Amanda L. ;
Davis, C. Todd ;
Saad, Magdi D. ;
El-Sayed, Nasr ;
Esmat, Hala ;
Tjaden, Jeffrey A. ;
Earhart, Kenneth C. ;
Ahmed, Lu'ay E. ;
Abd El-Halem, Mohamed ;
Ali, Abdel Hakem M. ;
Nassif, Samir A. ;
El-Ebiary, Elham A. ;
Taha, M. ;
Aly, Mona M. ;
Arafa, Abdelstattar ;
O'Neill, Eduardo ;
Xu Xiyan ;
Cox, Nancy J. ;
Donis, Ruben O. ;
Klimov, Alexander I. .
AVIAN DISEASES, 2010, 54 (01) :329-334
[5]   CHARACTERIZATION OF VIRULENT AND AVIRULENT A-CHICKEN-PENNSYLVANIA-83 INFLUENZA-A VIRUSES - POTENTIAL ROLE OF DEFECTIVE INTERFERING RNAS IN NATURE [J].
BEAN, WJ ;
KAWAOKA, Y ;
WOOD, JM ;
PEARSON, JE ;
WEBSTER, RG .
JOURNAL OF VIROLOGY, 1985, 54 (01) :151-160
[6]   Continuing progress towards a unified nomenclature for the highly pathogenic H5N1 avian influenza viruses: divergence of clade 2.2 viruses [J].
Brown, Ian H. ;
Capua, Ilaria ;
Cattoli, Giovanni ;
Chen, Hualan ;
Cox, Nancy ;
Davis, C. Todd ;
Donis, Ruben O. ;
Fouchier, Ron A. M. ;
Garten, Rebecca ;
Guan, Yi ;
Hay, Alan ;
Kawaoka, Yoshihiro ;
Mackenzie, John ;
McCauley, John ;
Mumford, Elizabeth ;
Olsen, Christopher ;
Perdue, Michael L. ;
Russell, Colin A. ;
Smith, Catherine ;
Smith, Derek ;
Smith, Gavin J. D. ;
Shu, Yuelong ;
Tashiro, Masato ;
Vijaykrishna, Dhanasekaran ;
Webster, Robert .
INFLUENZA AND OTHER RESPIRATORY VIRUSES, 2009, 3 (02) :59-62
[7]  
Brugh M, 1986, P 2 INT S AV INFL US, P283
[8]  
CAPPUCCI DT, 1985, AVIAN DIS, V29, P1195, DOI 10.2307/1590473
[10]   Highly Pathogenic Avian Influenza Virus Infection of Mallards with Homo- and Heterosubtypic Immunity Induced by Low Pathogenic Avian Influenza Viruses [J].
Fereidouni, Sasan R. ;
Starick, Elke ;
Beer, Martin ;
Wilking, Hendrik ;
Kalthoff, Donata ;
Grund, Christian ;
Haeuslaigner, Rafaela ;
Breithaupt, Angele ;
Lange, Elke ;
Harder, Timm C. .
PLOS ONE, 2009, 4 (08)