Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2-A Novel Magnetic Nanotube-Polyethylene Glycol Amine Composite-And Its Applications for Biodiesel Production

被引:19
作者
Abdulmalek, Saadiah A. [1 ,2 ]
Li, Kai [1 ]
Wang, Jianhua [1 ]
Ghide, Michael Kidane [1 ,3 ]
Yan, Yunjun [1 ]
机构
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Peoples R China
[2] Sanaa Univ, Fac Sci, Dept Biol, Sanaa 1247, Yemen
[3] Mainefhi Coll Sci, Eritrea Inst Technol, Dept Biol, Mainefhi 12676, Eritrea
基金
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词
biodiesel; co-immobilization; 4-arm-PEG-NH2; lipase; ultrasound-assisted enzymatic reaction; BURKHOLDERIA-CEPACIA LIPASE; RHIZOMUCOR-MIEHEI LIPASE; CATALYZED TRANSESTERIFICATION; ENZYMATIC TRANSESTERIFICATION; ORGANIC-SOLVENT; JATROPHA OIL; PALM OIL; IMMOBILIZATION; INTENSIFICATION; OPTIMIZATION;
D O I
10.3390/ijms222111956
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This article describes the successful synthesis of a novel nanocomposite of superparamagnetic multi-walled nanotubes with a four-arm polyethylene glycol amine polymer (mMWCNTs@4-arm-PEG-NH2). This composite was then employed as a support for the covalent co-immobilization of Rhizopus oryzae and Candida rugosa lipases under appropriate conditions. The co-immobilized lipases (CIL-mMWCNTs@4-arm-PEG-NH2) exhibited maximum specific activity of 99.626U/mg protein, which was 34.5-fold superior to that of free ROL, and its thermal stability was greatly improved. Most significantly, CIL-mMWCNTs@4-arm-PEG-NH2 was used to prepare biodiesel from waste cooking oil under ultrasound conditions, and within 120 min, the biodiesel conversion rate reached 97.64%. This was due to the synergy effect between ROL and CRL and the ultrasound-assisted enzymatic process, resulting in an increased biodiesel yield in a short reaction time. Moreover, after ten reuse cycles, the co-immobilized lipases still retained a biodiesel yield of over 78.55%, exhibiting excellent operational stability that is attractive for practical applications. Consequently, the combined use of a novel designed carrier, the co-immobilized lipases with synergy effect, and the ultrasound-assisted enzymatic reaction exhibited potential prospects for future applications in biodiesel production and various industrial applications.
引用
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页数:26
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