Antimicrobial chitosan nanodroplets: new insights for ultrasound-mediated adjuvant treatment of skin infection

被引：0

作者：

Banche, Giuliana ^{[1
]}

Prato, Mauro ^{[1
,2
]}

Magnetto, Chiara ^{[3
]}

Allizond, Valeria ^{[1
]}

Giribaldi, Giuliana ^{[4
]}

Argenziano, Monica ^{[5
]}

Khadjavi, Amina ^{[2
]}

Gulino, Giulia Rossana ^{[4
]}

Finesso, Nicole ^{[4
]}

Mandras, Narcisa ^{[1
]}

Tullio, Vivian ^{[1
]}

Cavalli, Roberta ^{[5
]}

Guiot, Caterina ^{[2
]}

Cuffini, Anna Maria ^{[1
]}

机构：

[1] Univ Torino, Dipartimento Sci Sanita Pubbl & Pediat, Turin, Italy

[2] Univ Torino, Dipartimento Neurosci, Turin, Italy

[3] Ist Nazl Ric Metrol INRIM, Turin, Italy

[4] Univ Torino, Dipartimento Oncol, Turin, Italy

[5] Univ Torino, Dipartimento Sci & Tecnol Farmaco, Turin, Italy

来源：

FUTURE MICROBIOLOGY | 2015年 / 10卷 / 06期

关键词：

Candida albicans; chitosan; MRSA; nanodroplet; oxygen; skin; KERATINOCYTE CELL-LINE; PORCINE EAR SKIN; OXYGEN DELIVERY; IN-VITRO; NANOPARTICLES; MODEL; NANOTECHNOLOGY; BIOFILMS; AGENTS; VIVO;

D O I：

10.2217/FMB.15.27

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Chronic wounds, characterized by hypoxia, inflammation and impaired tissue remodeling, are often worsened by bacterial/fungal infections. Intriguingly, chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLNs) have proven effective in delivering oxygen to hypoxic tissues. Aim: The present work aimed at investigating nanodroplet antimicrobial properties against methicillin-resistant Staphylococcus aureus (MRSA) or Candida albicans, toxicity on human keratinocytes (HaCaT) and ultrasound (US)-triggered transdermal delivery. Materials & methods: Nanodroplet antibacterial/antifungal properties, human cytotoxicity, and US-triggered transdermal delivery were measured through microbiological, biochemical, and sonophoresis assays, respectively. Results: OLNs and oxygen-free nanodroplets (OFNs) displayed short- or longterm cytostatic activity against MRSA or Candida albicans, respectively. OLNs were not toxic to keratinocytes, whereas OFNs slightly affected cell viability. Complementary US treatment promoted OLN transdermal delivery. Conclusion: As such, US-activated chitosan-shelled OLNs appear as promising, nonconventional and innovative tools for adjuvant treatment of infected chronic wounds.

引用

页码：929 / 939

页数：11

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