Development of a CHO cell line for stable production of recombinant antibodies against human MMP9

被引：8
作者：
Ryu, Jina [1 ]
Kim, Eun-Jung [2 ]
Kim, Joo-Kyung [1 ]
Park, Tai Hyun [1 ,2 ,3 ]
Kim, Byung-Gee [1 ,2 ,3 ]
Jeong, Hee-Jin [4 ]
机构：
[1] Seoul Natl Univ, Interdisciplinary Program Bioengn, Seoul 08826, South Korea
[2] Seoul Natl Univ, Inst Chem Proc, Sch Chem & Biol Engn, Seoul 08826, South Korea
[3] Seoul Natl Univ, BioMAX N Bio Inst, Inst Bioengn, Seoul 08826, South Korea
[4] Hongik Univ, Dept Biol & Chem Engn, Sejong 30016, South Korea
来源：
BMC BIOTECHNOLOGY | 2022年 / 22卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
Matrix metalloproteinase 9; Recombinant antibody; CHO cell line; MONOCLONAL-ANTIBODIES; GELATINASE B; PROTEIN; MATRIX-METALLOPROTEINASE-9;
D O I：
10.1186/s12896-022-00738-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamster ovary (CHO) cell line for the stable production of antibodies against hMMP9. Results Weconstructed recombinant anti-hMMP9 antibody fragment-expressing genes and transfected these to CHO cells. We chose a single clone, and successfully produced a full-sized antibody against hMMP9 with high purity, sensitivity, and reproducibility. Subsequently, we confirmed the antigen-binding efficiency of the antibody. Conclusions We developed a novel recombinant anti-hMMP9 antibody via a CHO cell-based mammalian expression system, which has a high potential to be used in a broad range of medical and industrial areas.
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页数：8
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