DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

被引：25

作者：

Mikheikin, Andrey ^{[1
]}

Olsen, Anita ^{[1
]}

Leslie, Kevin ^{[1
]}

Russell-Pavier, Freddie ^{[2
,3
]}

Yacoot, Andrew ^{[2
]}

Picco, Loren ^{[3
]}

Payton, Oliver ^{[3
]}

Toor, Amir ^{[4
,5
]}

Chesney, Alden ^{[5
,6
]}

Gimzewski, James K. ^{[7
]}

Mishra, Bud ^{[8
,9
]}

Reed, Jason ^{[1
,5
]}

机构：

[1] Virginia Commonwealth Univ, Dept Phys, Richmond, VA 23284 USA

[2] Natl Phys Lab, Hampton Rd, Teddington TW11 0LW, Middx, England

[3] Interface Anal Ctr, HH Wills Phys Lab, Tyndall Ave, Bristol BS8 1TL, Avon, England

[4] VCU Sch Med, Dept Internal Med, Richmond, VA 23284 USA

[5] VCU Massey Canc Ctr, Richmond, VA 23284 USA

[6] VCU Sch Med, Dept Pathol, Richmond, VA 23284 USA

[7] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[8] NYU, Courant Inst Math Sci, Dept Comp Sci, New York, NY 10012 USA

[9] NYU, Courant Inst Math Sci, Dept Math, New York, NY 10012 USA

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

英国工程与自然科学研究理事会; 美国国家卫生研究院;

关键词：

ATOMIC-FORCE MICROSCOPY; FOLLICULAR LYMPHOMA; GENOMIC DNA; BRCA1; MICA; BREAST; CANCER; GRADE; GENE; PCR;

D O I：

10.1038/s41467-017-01891-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.

引用

页数：9

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