Application of Optical Biosensors in Small-Molecule Screening Activities

被引：42
作者：
Geschwindner, Stefan [1 ]
Carlsson, Johan F. [1 ]
Knecht, Wolfgang [2 ]
机构：
[1] AstraZeneca R&D, Discovery Sci, S-43183 Molndal, Sweden
[2] AstraZeneca R&D, CVGI iMed, Biosci, S-43183 Molndal, Sweden
来源：
SENSORS | 2012年 / 12卷 / 04期
关键词：
screening; fragment; ligand; biosensor; surface plasmon resonance; optical waveguide grating; drug discovery; BINDING; IDENTIFICATION; PROTEINS; ASSAYS;
D O I：
10.3390/s120404311
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms.
引用
收藏
页码：4311 / 4323
页数：13
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