Identification of the cis-acting elements involved in regulation of xylanase III gene expression in Trichoderma reesei PC-3-7

被引：23

作者：

Furukawa, Takanori ^{[1
]}

Shida, Yosuke ^{[1
]}

Kitagami, Naoki ^{[1
]}

Ota, Yuuki ^{[1
]}

Adachi, Masahiro ^{[1
]}

Nakagawa, Shiho ^{[1
]}

Shimada, Ryuichi ^{[1
]}

Kato, Masashi ^{[2
]}

Kobayashi, Tetsuo ^{[2
]}

Okada, Hirofumi ^{[1
]}

Ogasawara, Wataru ^{[1
]}

Morikawa, Yasushi ^{[1
]}

机构：

[1] Nagaoka Univ Technol, Dept Bioengn, Niigata 9402188, Japan

[2] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Aichi 4648601, Japan

来源：

FUNGAL GENETICS AND BIOLOGY | 2008年 / 45卷 / 07期

关键词：

Trichoderma reesei; Hypocrea jecorina; xyn3; xylanase; cellulase; transcriptional regulation; Xyr1;

D O I：

10.1016/j.fgb.2008.03.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The xylanase III gene (xyn3) from the filamentous fungus Trichoderma reesei PC-3-7 is only induced by cellulose, its derivatives and L-sorbose, but not by xylan. In this study, we defined three cis-acting elements within the xyn3 upstream region by using detailed deletion and mutation analysis. In addition to the Xyr1/ACEII-binding motif (5'-GGCTAA-3'), the analogous motifs 5'-GGCTAT-3' and 5'-GGCAAA-3' presented as an inverted repeat spaced by 16-bp internal sequences were identified as essential elements for xyn3 expression. Electrophoretic mobility shift assay using heterologously expressed Xyr1 demonstrates that all the identified cis-acting elements are able to interact with Xyr1. Furthermore, no xyn3 transcripts were formed in the xyr1-knockout strain upon induction by sophorose and L-sorbose. These results indicate that xyn3 expression is transcriptionally regulated by Xyr1, and suggest that the 5'-GGCTAT-3' and 5'-GGCAAA-3' motifs play roles in Xyr1-mediated cellulase and xylanase gene expression in T. reesei. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：1094 / 1102

页数：9

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