Artin M enhances TNF-α production and phagocytosis of Candida albicans mediated by dectin-1 and mannose receptors

The activities of dectin-1 and mannose receptors on phagocytosis of Candida albicans and the production of TNF-alpha by macrophages from mice pretreated for 3 days with extract of Artocarpus intergrifolia seeds (jack extract), Artin M or jacalin were studied. Macrophages from these mice were coincubated with C albicans CR15 (yeast), in the presence of mannose (50 mM) plus mannan (100 mu g) or laminarin (1 mg). Phagocytosis was significantly enhanced to 52% in macrophages from mice pretreated intraperitoneally for 3 days with jack extract (500 mu g/250 mu l PBS). Reduction in phagocytosis from 52% to 34% (P<0.05) occurred in the presence of mannose receptor inhibitors and from 52% to 16% (P<0.01) in the presence of dectin-1 inhibitor laminarin, whereas only 20% of control macrophages phagocytosed blastoconidia. Similar results were verified for pretreatment of mice with Artin M (2.5 mu g/250 mu l PBS), but not for jacalin (25 mu g/250 mu l PBS). Macrophages from mice pretreated 3 days previously with jack extract or Artin M and then coincubated for 2 h with C. albicans presented a significant increase in TNF-alpha production, correlating with significantly less transition of yeast to filamentous forms compared to pretreatment with jacalin. These results suggest that Artin M. but not jacalin present in jack extract significantly increased TNF-alpha production and the activity of mannose and dectin-1 receptors. (C) 2011 Elsevier B.V. All rights reserved.