Effects of propofol and sevoflurane on tumor killing activity of peripheral blood natural killer cells in patients with gastric cancer

被引：23
作者：
Ai, Lili [1 ,2 ]
Wang, Hao [1 ]
机构：
[1] Jinzhou Med Univ, Affiliated Hosp 1, Dept Anesthesiol, 2,Sect 5,Renmin St, Jinzhou 121000, Liaoning, Peoples R China
[2] Jinzhou Med Univ, Affiliated Hosp 3, Dept Anesthesiol, Jinzhou, Liaoning, Peoples R China
来源：
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2020年 / 48卷 / 03期
关键词：
Propofol; sevoflurane; tumor killing activity; peripheral blood natural killer cells; gastric cancer; cytotoxicity; SMAD4; PERIOPERATIVE PERIOD; BREAST-CANCER; TGF-BETA; NK CELLS; ANESTHESIA; IMMUNOTHERAPY; SUFENTANIL; EXPRESSION; RECEPTOR; COLON;
D O I：
10.1177/0300060520904861
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective This study aimed to investigate the effects of propofol and sevoflurane on cytotoxicity of natural killer (NK) cells in patients with gastric cancer. Methods Patients with gastric cancer were anesthetized by propofol or sevoflurane. Peripheral blood NK cells were isolated and co-cultured with BGC-823 gastric cancer cell culture supernatant, and the rate of apoptosis and effector molecules were analyzed by flow cytometry. Effects of propofol and sevoflurane on NK cell function and SMAD4 protein expression were investigated. Results Cytotoxicity of NK cells in patients with gastric cancer was inhibited before surgery, but it was enhanced in patients who were anesthetized by propofol compared with those who had sevoflurane. In vitro co-culture with BGC-823 cells significantly inhibited the cytotoxicity of NK cells, which was abolished by treatment of propofol or transforming growth factor (TGF)-beta 1. SMAD4 protein expression in the NK cell nucleus was significantly downregulated by TGF-beta 1 treatment and BGC-823 supernatant co-culture, and this expression could be restored by propofol. Conclusions Cytotoxicity of NK cells in patients with gastric cancer is low, but it can be promoted by propofol. Propofol regulates cytotoxicity in NK cells by promoting SMAD4, thereby affecting cellular function.
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页数：15
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