Population genetic variation of the Southern Ocean krill, Euphausia superba, in the Western Antarctic Peninsula region based on mitochondrial single nucleotide polymorphisms (SNPs)

被引:17
作者
Batta-Lona, Paola G. [1 ]
Bucklin, Ann [1 ]
Wiebe, Peter H. [2 ]
Patarnello, Tomaso [3 ]
Copley, Nancy J. [2 ]
机构
[1] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA
[2] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA
[3] Univ Padua, Dept Publ Hlth Comparat Pathol & Vet Hyg, I-35100 Padua, Italy
基金
美国国家科学基金会;
关键词
Euphausia superba; Population genetics; Single nucleotide polymorphism; Southern Ocean krill; AUSTRAL FALL; DANA; VARIABILITY; IDENTIFICATION; DYNAMICS; WINTER; DIFFERENTIATION; HYDROGRAPHY; CIRCULATION; CURRENTS;
D O I
10.1016/j.dsr2.2010.11.017
中图分类号
P7 [海洋学];
学科分类号
0707 ;
摘要
The Southern Ocean krill, Euphausia superba, is one of the best-studied marine zooplankton species in terms of population genetic diversity and structure; with few exceptions, previous studies have shown the species to be genetically homogeneous at larger spatial scales. The goals of this study are to examine sub-regional scale population genetic diversity and structure of E. superba using molecular characters selected with this goal in mind, and to thereby examine hypotheses of the source(s) of recruitment for krill populations of the Western Antarctic Peninsula (WAP). Collections were made throughout the WAP region during US GLOBEC cruises in austral fall, 2001 and 2002. A total of 585 E. superba (including all 6 furcilia larval stages, juveniles, and adults) was analyzed after confirmation of species identification using a competitive multiplexed species-specific PCR (SS-PCR) reaction based on mitochondrial cytochrome oxidase I (mtCOI) sequences. The molecular markers used were allele frequencies at single nucleotide polymorphism (SNP) sites in the gene encoding mitochondria! Cytochrome b (cyt 6). Four SNP sites that showed desirable patterns of allelic variation were selected; alleles were detected using a multiplexed single-base extension PCR protocol. A total of 22 SNP haplotypes (i.e., strings of polymorphisms at the four SNP sites) was observed; haplotype diversity (Hd)=0.811 (s.d.=0.008). Analysis of molecular variation within and among samples, areas (i.e., Marguerite Bay, Crystal Sound, shelf, and offshore) and collection years revealed no difference between 2001 and 2002 collections overall, although differences between 2001 and 2002 collections from Marguerite Bay explained 7.4% of the variance (F-ST=0.072; p=0.002 +/- 0.001). Most of the variation (96.3%) occurred within samples each year, with no significant differentiation among areas. There was small, but significant differentiation among samples within areas in 2001 (4.6%; F-ST=0.045; p=0.015 +/- 0.003) and 2002 (6.3%; F-ST=0.062; p=0.000 +/- 0.000). There was evidence of life stage-specific spatial differentiation for furcilia in 2002 for F1 (18.1%, p=0.000 +/- 0.000) and F2 (9.2%, p=0.001 +/- 0.001). The significant differentiation among samples for E. superba within areas was interpreted as evidence of multiple sources of recruitment of E. superba in the WAP region, consistent with advective transport in observed circulation patterns and reproduction and spawning in both offshore and shelf habitats. Further population genetic analysis at sub-regional scales is needed to understand and eventually predict population dynamic processes (e.g., recruitment, migration, retention, and over-wintering) of Southern Ocean krill. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1652 / 1661
页数:10
相关论文
共 81 条
[1]   Distribution of larval krill and zooplankton in association with hydrography in Marguerite Bay, Antarctic Peninsula, in austral fall and winter 2001 described using the Video Plankton Recorder [J].
Ashjian, Carin J. ;
Davis, Cabell S. ;
Gallager, Scott M. ;
Wiebe, Peter H. ;
Lawson, Gareth L. .
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2008, 55 (3-4) :455-471
[2]   Oceanic circumpolar habitats of Antarctic krill [J].
Atkinson, A. ;
Siegel, V. ;
Pakhomov, E. A. ;
Rothery, P. ;
Loeb, V. ;
Ross, R. M. ;
Quetin, L. B. ;
Schmidt, K. ;
Fretwell, P. ;
Murphy, E. J. ;
Tarling, G. A. ;
Fleming, A. H. .
MARINE ECOLOGY PROGRESS SERIES, 2008, 362 :1-23
[3]   Long-term decline in krill stock and increase in salps within the Southern Ocean [J].
Atkinson, A ;
Siegel, V ;
Pakhomov, E ;
Rothery, P .
NATURE, 2004, 432 (7013) :100-103
[4]   South Georgia, Antarctica: a productive, cold water, pelagic ecosystem [J].
Atkinson, A ;
Whitehouse, MJ ;
Priddle, J ;
Cripps, GC ;
Ward, P ;
Brandon, MA .
MARINE ECOLOGY PROGRESS SERIES, 2001, 216 :279-308
[5]  
Avise J. C., 2000, PHYLOGEOGRAPHY HIST, DOI DOI 10.2307/J.CTV1NZFGJ7
[6]  
Avise John C., 1994, pi
[7]   GENETIC-VARIABILITY IN THE PELAGIC ENVIRONMENT - PARADOX [J].
AYALA, FJ ;
VALENTINE, JW .
ECOLOGY, 1979, 60 (01) :24-29
[8]   Median-joining networks for inferring intraspecific phylogenies [J].
Bandelt, HJ ;
Forster, P ;
Röhl, A .
MOLECULAR BIOLOGY AND EVOLUTION, 1999, 16 (01) :37-48
[9]   DISTRIBUTION AND POPULATION STRUCTURES OF IMMATURE AND ADULT EUPHAUSIA-SUPERBA IN THE WESTERN BRANSFIELD STRAIT REGION DURING THE 1986-87 SUMMER [J].
BRINTON, E .
DEEP-SEA RESEARCH PART A-OCEANOGRAPHIC RESEARCH PAPERS, 1991, 38 (8-9) :1169-1193
[10]   Gene flow patterns of the euphausiid, Meganyctiphanes norvegica, in the NW Atlantic based on mtDNA sequences for cytochrome b and cytochrome oxidase I [J].
Bucklin, A ;
Smolenack, SB ;
Bentley, AM ;
Wiebe, PH .
JOURNAL OF PLANKTON RESEARCH, 1997, 19 (11) :1763-1781