Nanoscale Analysis of Glycolipid Distribution in the Cell Membrane

被引：0

作者：

Fujita, Akikazu ^{[1
]}

Fujimoto, Toyoshi ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Anat & Mol Cell Biol, Nagoya, Aichi 4668550, Japan

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2010年 / 22卷 / 126-28期

关键词：

ganglioside; phosphatidylinositol 4,5-bisphosphate; quick freezing; electron microscopy; freeze-fracture; FREEZE-FRACTURE REPLICA; SINGLE-MOLECULE TRACKING; GPI-ANCHORED PROTEINS; LIPID RAFTS; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; ELECTRON-MICROSCOPY; DOMAINS; RECEPTOR; MODEL; GANGLIOSIDES;

D O I：

10.4052/tigg.22.173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biological membranes at ambient temperature show fluidity in two dimensions. Both proteins and lipids, however, do not necessarily distribute evenly but often have a biased distribution. Methods to visualize membrane lipids are limited compared to those for membrane proteins, and distribution of endogenous lipids has been difficult to analyze quantitatively. We recently developed a method to immobilize membrane lipids physically by combining quick freezing and freeze-fracture techniques, whereby we could observe their two-dimensional distribution at the nanoscale. The method has a high capture ratio, and the point pattern analyses can be applied to the result. This article mainly discussed the results obtained for gangliosides GM1 and GM3 and also referred to the results for phosphatidylinositol 4,5-bisphosphate.

引用

页码：173 / 181

页数：9

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