Serotyping, genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum strains isolated from farmed rainbow trout (Oncorhynchus mykiss) in the eastern Black Sea

被引:1
作者
Balta, Fikri [1 ]
Dengiz Balta, Zeynep [1 ]
机构
[1] Recep Tayyip Erdogan Univ, Hastaliklar Anabilim Dali, Su Urunleri Fak, Zihniderin Yerleskesi,Fener Mah, TR-53100 Rize, Turkey
来源
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI | 2017年 / 64卷 / 04期
关键词
Antimicrobial sensitivity; diagnostic; PCR; rainbow trout; Vibrio anguillarum; MARINE FISH; IDENTIFICATION; LISTONELLA; DIAGNOSIS;
D O I
10.1501/Vetfak_0000002816
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study. The biochemical, serological and molecular techniques were used in identification of V. anguillarum isolates. The sequenced isolates were confirmed to be similar to V. anguillarum which stored under different accession numbers in GenBank database. The rate of 97-99% similarity were found among the compared isolates. All isolates were identified to be serotype O1 by slide agglutination test results. The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol.
引用
收藏
页码:321 / 328
页数:8
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