Genetic modification of essential fatty acids biosynthesis in Hansenula polymorpha

被引:19
作者
Laoteng, K [1 ]
Ruenwai, R
Tanticharoen, M
Cheevadhanarak, S
机构
[1] Kings Mongkuts Univ Technol, Natl Ctr Genet Engn & Biotechnol, Biochem Engn & Pilot Plant Res & Dev Unit, Bangkok 10150, Thailand
[2] King Mongkuts Univ Technol, Pilot Plant Dev & Training Inst, Bangkok 10150, Thailand
[3] King Mongkuts Univ Technol, Sch Bioresources & Technol, Bangkok 10150, Thailand
关键词
essential fatty acid; Delta(6)-desaturase; Mucor rouxii; Hansenula polymorpha;
D O I
10.1016/j.femsle.2005.03.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Delta(6)-desaturase gene isoform II involved in the formation of gamma-linolenic acid (GLA) was identified from Mucor rouxii. To study the possibility of alteration of the synthetic pathway of essential fatty acids in the methylotrophic yeast, Hansenula polymorpha, the cloned gene of M. rouxii under the control of the methanol oxidase (MOX) promoter of H. polymorpha, was used for genetic modification of this yeast. Changes in flux through the n-3 and n-6 pathways in the transgenic yeast were observed. The proportion of GLA varied dramatically depending on the growth temperature and media composition. This can be explained by the effects of either substrate availability or enzymatic activity. In addition to the potential application for manipulating the fatty acid profile, this study provides an attractive model system of H. polymorpha for investigating the deviation of fatty acid metabolism in eukaryotes. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:169 / 178
页数:10
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