Determination of phytic acid in feeds and faeces of pigs and poultry by capillary isotachophoresis

A method for phytic acid determination in the feeds and faeces of pigs and poultry has been developed on the basis of capillary isotachophoresis. Phytic acid was extracted by 0.95 M HCl and separated from interfering compounds by iron precipitation. Complete formation of ferric phytate required 7 mol FeCl(3)mol(-1) phytic acid. Residual Fe3+ was estimated colorimetricalty by the tiron reagent, and ferric phytate was dissolved in 1.5 M NaOH at 9 mol NaOH mol(-1) Fe precipitated. Analyses were carried out using an electrolyte system with Cl- as the leading anion, bis-tris-propane, and 2-morpholinoethanesulphonic acid as the terminating anion. The recovery of phytic acid (added to hen faeces) using this procedure was 962+/-24 g kg(-1) The limit of determination of phytic acid was 0.3 mu molml(-1) extract. The amount of phytic acid in feeds ranged from 8.3 to 10.88 kg(-1) on a dry matter basis. Phytic acid P represented 112g kg(-1) total P in faeces of young pigs (40-60 kg) fed a feed with supplemental phytase (490 Ukg(-1)), 153 g kg(-1) total P in faeces of finishing pigs and 185 g kg(-1) total P in faeces of non-lactating sows. Excreta of laying hens contained 23.7 g phytic acid kg(-1) dry matter (362 g kg(-1) total P). The isotachophoretic method is sufficiently simple and reproducible to be used for routine analyses of feeds and faeces. (C) 2000 Society of Chemical Industry.