PELDOR study on the tyrosyl radicals in the R2 protein of mouse ribonucleotide reductase

被引：19

作者：

Biglino, D ^{[1
]}

Schmidt, PP ^{[1
]}

Reijerse, EJ ^{[1
]}

Lubitz, W ^{[1
]}

机构：

[1] Max Planck Inst Bioinorgan Chem, D-45470 Mulheim, Germany

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2006年 / 8卷 / 01期

关键词：

D O I：

10.1039/b513950c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Pulse electron - electron double resonance (PELDOR) has been employed to measure the distance between the putative tyrosyl radicals in the two halves of the R2 subunit from mouse ribonucleotide reductase. The results provide experimental evidence that the active, tyrosyl radical containing mouse R2 subunit forms a homodimeric form in solution. The distance between the two tyrosyl radicals present in the dimer was determined to be 3.25 +/- 0.05 nm.

引用

页码：58 / 62

页数：5

共 50 条

[21] STRUCTURE AND FUNCTION OF THE ESCHERICHIA-COLI RIBONUCLEOTIDE REDUCTASE PROTEIN R2 [J].

NORDLUND, P ;

EKLUND, H .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (01) :123-164

[22] KINETIC-STUDIES ON THE REDUCTION OF THE TYROSYL RADICAL OF THE R2 SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEOTIDE REDUCTASE [J].

SWARTS, JC ;

AQUINO, MAS ;

HAN, JY ;

LAM, KY ;

SYKES, AG .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1247 (02) :215-224

[23] X-ray and spectroscopic studies of dinuclear sites in ribonucleotide reductase R2 from mouse show new carboxylate and tyrosyl radical [J].

Andersson, KK ;

Strand, KR ;

Karlsen, S ;

Cederkvist, FH ;

Görbitz, CH .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2003, 96 (01) :91-91

[24] Loss of the tyrosyl radical in mouse ribonucleotide reductase by (-)-epicatechin [J].

Schroeder, P ;

Voevodskaya, N ;

Klotz, LO ;

Brenneisen, P ;

Gräslund, A ;

Sies, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 326 (03) :614-617

[25] REDUCTION OF THE TYROSYL RADICAL AND THE IRON CENTER IN PROTEIN R2 OF RIBONUCLEOTIDE REDUCTASE FROM MOUSE, HERPES-SIMPLEX VIRUS AND ESCHERICHIA-COLI BY P-ALKOXYPHENOLS [J].

POTSCH, S ;

SAHLIN, M ;

LANGELIER, Y ;

GRASLUND, A ;

LASSMANN, G .

FEBS LETTERS, 1995, 374 (01) :95-99

[26] REACTION OF NO WITH THE REDUCED R2 PROTEIN OF RIBONUCLEOTIDE REDUCTASE FROM ESCHERICHIA-COLI [J].

HASKIN, CJ ;

RAVI, N ;

LYNCH, JB ;

MUNCK, E ;

QUE, L .

BIOCHEMISTRY, 1995, 34 (35) :11090-11098

[27] Crystal structural studies of changes in the native dinuclear iron center of ribonucleotide reductase protein R2 from mouse [J].

Strand, KR ;

Karlsen, S ;

Kolberg, M ;

Rohr, ÅK ;

Görbitz, CH ;

Andersson, KK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (45) :46794-46801

[28] Resonance Raman evidence for a hydrogen-bonded oxo bridge in the R2 protein of ribonucleotide reductase from mouse [J].

Hanson, MA ;

Schmidt, PP ;

Strand, KR ;

Gräslund, A ;

Solomon, EI ;

Andersson, KK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (28) :6755-6756

[29] Resonance raman evidence for a Hydrogen-bonded oxo bridge in the R2 protein of ribonucleotide reductase from mouse [J].

Hanson, Melissa A. ;

Schmidt, Peter P. ;

Strand, Kari R. ;

Graeslund, Astrid ;

Solomon, Edward I. ;

Andersson, Kristoffer K. .

Journal of the American Chemical Society, 121 (28)

[30] Photoproduction of tyrosyl radicals for the study of proton-coupled electron transfer in ribonucleotide reductase [J].

Reece, SY ;

Seyedsayamdost, MR ;

Stubbe, J ;

Nocera, DG .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 :U2027-U2027

← 1 2 3 4 5 →