Methods of uniform differential item functioning (DIF) detection have been extensively studied in the complete data case. However, less work has been done examining the performance of these methods when missing item responses are present. Research that has been done in this regard appears to indicate that treating missing item responses as incorrect can lead to inflated Type I error rates (false detection of DIF). The current study builds on this prior research by investigating the utility of multiple imputation methods for missing item responses, in conjunction with standard DIF detection techniques. Results of the study support the use of multiple imputation for dealing with missing item responses. The article concludes with a discussion of these results for multiple imputation in conjunction with other research findings supporting its use in the context of item parameter estimation with missing data.