Cloning and functional characterization of the human VIP1/PACAP receptor promoter

被引：4

作者：

Couvineau, A ^{[1
]}

Maoret, JJ ^{[1
]}

Rouyer-Fessard, C ^{[1
]}

Carrero, I ^{[1
]}

Laburthe, M ^{[1
]}

机构：

[1] Fac Med Xavier Bichat, INSERM, U410, Lab Neuroendocrinol & Biol Cellulaire Digest, F-75870 Paris, France

来源：

VIP, PACAP, AND RELATED PEPTIDES: THIRD INTERNATIONAL SYMPOSIUM | 1998年 / 865卷

关键词：

D O I：

10.1111/j.1749-6632.1998.tb11163.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 5'-flanking region (1.5 kb) of the gene coding for the human VIP1/PACAP receptor was isolated, sequenced, and characterized. Transient expression of constructs containing sequentially deleted 5'-flanking sequences of the VIP1/PACAP receptor fused to a luciferase reporter gene showed that this sequence was active as a promoter in the intestinal cancer cell line, HT-29, expressing endogenous VIP1/PACAP receptor. The shortest DNA fragment with significant promoter activity encompassed the region from -205 to +76 bp. Deletion of a CCAAT-box sequence in the construction corresponding to -173 to +76 bp dramatically reduced the promoter activity. The promoter -205 to +76 bp has a housekeeping gene structure without TATA box. It contains GC-rich regions characterized by potential Sp1 and AP2 sites and some potential regulatory elements, such as CRE and ATF, and a CCAAT box sequence (-182 to -178) crucial for gene transcription.

引用

页码：59 / 63

页数：5

共 15 条

[1] Mutagenesis of N-glycosylation sites in the human vasoactive intestinal peptide 1 receptor. Evidence that asparagine 58 or 69 is crucial for correct delivery of the receptor to plasma membrane [J].

Couvineau, A ;

Fabre, C ;

Gaudin, P ;

Maoret, JJ ;

Laburthe, M .

BIOCHEMISTRY, 1996, 35 (06) :1745-1752

[2] Vasoactive intestinal peptide (VIP)(1) receptor - Three nonadjacent amino acids are responsible for species selectivity with respect to recognition of peptide histidine isoleucineamide [J].

Couvineau, A ;

RouyerFessard, C ;

Maoret, JJ ;

Gaudin, P ;

Nicole, P ;

Laburthe, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) :12795-12800

[3] HUMAN INTESTINAL VIP RECEPTOR - CLONING AND FUNCTIONAL EXPRESSION OF 2 CDNA-ENCODING PROTEINS WITH DIFFERENT N-TERMINAL DOMAINS [J].

COUVINEAU, A ;

ROUYERFESSARD, C ;

DARMOUL, D ;

MAORET, JJ ;

CARRERO, I ;

OGIERDENIS, E ;

LABURTHE, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 200 (02) :769-776

[4] MOLECULAR-IDENTIFICATION AND STRUCTURAL REQUIREMENT OF VASOACTIVE INTESTINAL PEPTIDE (VIP) RECEPTORS IN THE HUMAN-COLON ADENOCARCINOMA CELL-LINE, HT-29 [J].

COUVINEAU, A ;

ROUSSET, M ;

LABURTHE, M .

BIOCHEMICAL JOURNAL, 1985, 231 (01) :139-143

[5] HIGHLY CONSERVED ASPARTATE-68, TRYPTOPHANE-73 AND GLYCINE-109 IN THE N-TERMINAL EXTRACELLULAR DOMAIN OF THE HUMAN VIP RECEPTOR ARE ESSENTIAL FOR ITS ABILITY TO BIND VIP [J].

COUVINEAU, A ;

GAUDIN, P ;

MAORET, JJ ;

ROUYERFESSARD, C ;

NICOLE, P ;

LABURTHE, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 206 (01) :246-252

[6]

FAHRENKRUG J, 1989, HDB PHYSL GASTROINTE, V2, P611

[7] MUTATIONAL ANALYSIS OF CYSTEINE RESIDUES WITHIN THE EXTRACELLULAR DOMAINS OF THE HUMAN VASOACTIVE-INTESTINAL-PEPTIDE (VIP) 1-RECEPTOR IDENTIFIES 7 MUTANTS THAT ARE DEFECTIVE IN VIP BINDING [J].

GAUDIN, P ;

COUVINEAU, A ;

MAORET, JJ ;

ROUYERFESSARD, C ;

LABURTHE, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 211 (03) :901-908

[8]

LABURTHE M, 1987, J BIOL CHEM, V262, P10180

[9]

LABURTHE M, 1989, HDB PHYSL 6, V2, P615

[10] MOLECULAR-CLONING OF THE GENE ENCODING THE MOUSE PARATHYROID HORMONE/PARATHYROID HORMONE-RELATED PEPTIDE RECEPTOR [J].

MCCUAIG, KA ;

CLARKE, JC ;

WHITE, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :5051-5055

← 1 2 →