Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus

被引：25

作者：

Ueda, Tomofumi ^{[1
]}

Kaito, Chikara ^{[1
]}

Omae, Yosuke ^{[1
]}

Sekimizu, Kazuhisa ^{[1
]}

机构：

[1] Univ Tokyo, Microbiol Lab, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

MICROBIAL PATHOGENESIS | 2011年 / 51卷 / 03期

关键词：

Staphylococcus aureus; Colony spreading; Virulence; Catabolite repression; agr; CARBON CATABOLITE REPRESSION; BACILLUS-SUBTILIS; MYCOBACTERIUM-SMEGMATIS; SIGNAL-TRANSDUCTION; SLIDING MOTILITY; POTASSIUM-ION; VIRULENCE; CCPA; PROTEIN; METABOLISM;

D O I：

10.1016/j.micpath.2011.04.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Staphylococcus aureus spreads on soft agar surfaces, which is called "colony spreading". Here, we report that the colony spreading in S. aureus was promoted by the addition of glucose to soft agar plates. Disruption of ccpA and hprK, which are involved in catabolite repression, decreased the colony spreading ability promoted by glucose. Deletion of the agr locus, a virulence regulatory element whose expression is activated by glucose in a ccpA-dependent manner, abolished the colony spreading promoted by glucose. Disruption of clpP and arlRS, which contributes to agr expression, also decreased glucose-promoted colony spreading. These findings suggest that S. aureus colony spreading requires the expression of agr, which is positively regulated by environmental carbon sources, and that virulence gene expression and colony spreading induced by agr are simultaneously activated in the S. aureus infectious process. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：178 / 185

页数：8

共 45 条

[1]

[Anonymous], 1989, Molecular Cloning: A Laboratory

[2] Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis:: regulation of the central metabolic pathways [J].

Blencke, HM ;

Homuth, G ;

Ludwig, H ;

Mäder, U ;

Hecker, M ;

Stülke, J .

METABOLIC ENGINEERING, 2003, 5 (02) :133-149

[3] agr-mediated dispersal of Staphylococcus aureus biofilms [J].

Boles, Blaise R. ;

Horswill, Alexander R. .

PLOS PATHOGENS, 2008, 4 (04)

[4] Role of swarming migration in the pathogenesis of Bacillus endophthalmitis [J].

Callegan, Michelle C. ;

Novosad, Billy D. ;

Ramirez, Raul ;

Ghelardi, Emilia ;

Senesi, Sonia .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2006, 47 (10) :4461-4467

[5] Community-associated meticillin-resistant Staphylococcus aureus [J].

DeLeo, Frank R. ;

Otto, Michael ;

Kreiswirth, Barry N. ;

Chambers, Henry F. .

LANCET, 2010, 375 (9725) :1557-1568

[6] Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era [J].

DeLeo, Frank R. ;

Chambers, Henry F. .

JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (09) :2464-2474

[7] P-Ser-HPr - a link between carbon metabolism and the virulence of some pathogenic bacteria [J].

Deutscher, J ;

Herro, R ;

Bourand, A ;

Mijakovic, I ;

Poncet, S .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1754 (1-2) :118-125

[8] How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria [J].

Deutscher, Josef ;

Francke, Christof ;

Postma, Pieter W. .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2006, 70 (04) :939-+

[9] Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci [J].

Dunman, PM ;

Murphy, E ;

Haney, S ;

Palacios, D ;

Tucker-Kellogg, G ;

Wu, S ;

Brown, EL ;

Zagursky, RJ ;

Shlaes, D ;

Projan, SJ .

JOURNAL OF BACTERIOLOGY, 2001, 183 (24) :7341-7353

[10] STAPHYLOCOCCAL COAGULASE - MODE OF ACTION AND ANTIGENICITY [J].

DUTHIE, ES ;

LORENZ, LL .

JOURNAL OF GENERAL MICROBIOLOGY, 1952, 6 (1-2) :95-107

← 1 2 3 4 5 →