Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts

被引：10
作者：
Sun, Fangfang [1 ]
Liu, Ying [2 ]
Pan, Yahui [1 ]
Chen, Meng [3 ]
Meng, Xiangfeng [1 ]
机构：
[1] Nanjing Univ, Nanjing Stomatol Hosp, Med Sch, Dept Prosthodont, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Affiliated Jiangning Hosp, Dept Stomatol, Nanjing, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Childrens Hosp, Dept Resp, Nanjing, Jiangsu, Peoples R China
来源：
BIOMED RESEARCH INTERNATIONAL | 2018年 / 2018卷
基金：
中国国家自然科学基金;
关键词：
CARBOXYLESTERASE EXPRESSION; CYCLOOXYGENASE-2; EXPRESSION; WATER SORPTION; BISGMA; DIMETHACRYLATE; DEGRADATION; SOLUBILITY; APOPTOSIS;
D O I：
10.1155/2018/7823467
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to evaluate the potential cytotoxicity of self-adhesive resin cements with or without light irradiation on human periodontal ligament fibroblasts (HPDLFs) in vitro. Three self-adhesive resin cements (RelyX U200, Maxcem Elite and Multilink Speed) were cured with light or not. Cured cements were stored at 37 degrees C for 24 h in water or Dulbecco's modified eagle medium (DMEM) medium. Their chromatographic analysis of water-based extract solution was made and then the DMEM-based extract solution was diluted in complete DMEM {1:5, 1:10, 1:20, 1:40, 1:80 (v/v)} for evaluating cell relative growth rate and cell apoptosis/necrosis rate of HPDLFs. The data was analyzed by one-way ANOVA and independent T test. Regardless of light irradiation, cell relative growth rate increased, and the apoptosis/necrosis rate of each resin cement decreased with the increase of gradient dilution. Regardless of gradient dilution, the cell relative growth rate and apoptosis/necrosis rate of RelyX U200 and Maxcem Elite with light irradiation were higher than those without light irradiation. Besides, without light irradiation, Multilink Speed showed higher cell relative growth rate and lower apoptosis/necrosis rate than other cements. Light irradiation and composition difference of self-adhesive resin cements could affect their cytotoxicity on HPDLFs.
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页数：10
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