Cross-linked protein crystals by glutaraldehyde and their applications

被引：68

作者：

Yan, Er-Kai ^{[1
]}

Cao, Hui-Ling ^{[1
,2
]}

Zhang, Chen-Yan ^{[1
]}

Lu, Qin-Qin ^{[1
]}

Ye, Ya-Jing ^{[1
]}

He, Jin ^{[1
]}

Huang, Lin-Jun ^{[1
]}

Yin, Da-Chuan ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Space Biotechnol, Inst Special Environm Biophys, Xian 710072, Shaanxi, Peoples R China

[2] Xian Med Univ, Inst Basic & Translat Med, Shaanxi Key Lab Ischem Cardiovasc Dis, Xian 710021, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 33期

基金：

中国国家自然科学基金;

关键词：

LYSOZYME SINGLE-CRYSTALS; X-RAY-DIFFRACTION; ENZYME CRYSTALS; ORGANIC-SOLVENTS; CHEMICAL-MODIFICATION; IMMOBILIZED ENZYMES; MAGNETIC-PROPERTIES; GOLD NANOPARTICLES; CARBOXYPEPTIDASE-A; ISOMERASE CRYSTALS;

D O I：

10.1039/c5ra01722j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cross-linked protein crystal technology, as either a protein stabilisation or enzyme immobilisation method, has garnered more attention recently. This method not only can retain the original activity of the protein molecule but can also significantly enhance the crystals' mechanical and chemical stability. This review presents the preparation and mechanism of cross-linked protein crystals using glutaraldehyde. The mechanical, chemical and thermal properties of the cross-linked protein crystals are also reviewed in detail. In addition, this paper summarises the applications of cross-linked protein crystals in the fields of materials science, biosensors, chromatographic analysis, oral delivery and protein crystal quality improvement. Finally, the limitations and perspectives on cross-linked protein crystals are presented.

引用

页码：26163 / 26174

页数：12

共 125 条

[1] Porous Protein Crystals as Reaction Vessels for Controlling Magnetic Properties of Nanoparticles [J].