2D or not 2D

被引：138

作者：

Fey, SJ ^{[1
]}

Larsen, PM ^{[1
]}

机构：

[1] Univ So Denmark, Ctr Proteome Anal, DK-5230 Odense M, Denmark

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(00)00167-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2D gel electrophoresis is the technology that everyone loves to hate - it requires manual dexterity and precision to reproduce precisely and is thus not well-suited as a high-throughput technology. Although almost everyone would like to replace it, the resolution and sensitivity it offers are exquisite and unsurpassed if one wants a global view of cellular activity. There have been several recent developments, for example, the detection of low abundance proteins, and the resolution possible with narrow-range IPG gels.

引用

页码：26 / 33

页数：8

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